碩士 / 國立成功大學 / 化學工程學系碩博士班 / 97 / Polyacrylonitrile (PAN) nanofibrous membrane providing huge surface area could be manufactured by electrospinning as support for enzyme immobilization. The enzyme, Aspergillus niger cellulase was immobilized on electrospun PAN nanofibrous membranes by amidination reaction for long term operation. In addition, covalent bond formation between enzyme molecule and the nanofiber was confirmed from FT-IR measurement. The optimal operation conditions of cellulase immobilization for the highest specific activity as the enzyme concentration of 2 wt%, immobilization time of 30 min, temperature at 40°C and pH value at 4.6. The immobilized cellulase onto PAN nanofibrous membranes was further employed to investigate the hydrolysis reaction of microalgal cellulose. The highest yields of reducing sugars and glucose were 62% and 50% respectively under the optimal conditions. Since microalgal compositions were not only cellulose but also lipid, the lipid can be extracted for other applications. After the enzymatic process, the yield of extraction was 1.75 times higher than that of unpretreated by cellulase. The observed results indicate that the enzymatic hydrolysis of microalgal cellulose has the potential industrial applications for producing reducing sugars.
| Identifer | oai:union.ndltd.org:TW/097NCKU5063125 |
| Date | January 2009 |
| Creators | Jing-Yi Chen, 陳靜儀 |
| Contributors | Wen-Teng Wu, 吳文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 83 |
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