碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 97 / Myostatin (GDF-8), which belongs to TGF-ß superfamily, is a secreted growth and differentiation factor. It functions as a negative regulator for the growth of skeletal muscle. In the functional analysis of Myostatin, the open reading frame is very conservative, including three domains: (1) N-terminus domain, it functions as secretory signal peptide, (2) conserved proteolytic processing site RXRR, and (3) C-terminus cysteine-rich region as mature bioactive region. Among them, C-terminus cysteine-rich region was determined the most important region. We developed an immunodepletion technique, which is sufficient to induce anti-Myostatin immunoresponse, to demonstrate the function of Myostatin in the grouper. In addition, orange-spotted grouper (Epinephelus coioides) myostatin promoter is defined as 1936-bp and contains ten putative E-Box (CANNTG) motifs. Various constructs of the grouper myostatin promoter were transfected into the grouper cells, regulatory regions on myostatin promoter and the relative regulatory factors were analysed.
| Identifer | oai:union.ndltd.org:TW/097NCKU5111011 |
| Date | January 2009 |
| Creators | Wan-erh Chen, 陳宛兒 |
| Contributors | Zong-yue Chen, 陳宗嶽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 107 |
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