碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 97 / Chitinase (EC 3.2.1.14) is a kind of glycosyl hydrolase which can hydrolysis chitin. Two chitinase genes (chitinase 1 and chitinase 2) have been cloned from the stomach of orange-spotted grouper (Epinephelus coioides). It is found that the chitinase was participating in the food digestions that contain the chitin in ingredient. In this study, four topics based on the previous data will be further discussing. The first is cloning the chitinase genomic DNA sequence. Second is to construct the phylogenetic tree of vertebrate chitinases. Third is to investigate the chitinase genes expression in the different development stages of the larvae. Fourth is to know the molecular mechanisms about the regulation of the chitinase genes expression. In the results, it could be found both of chitinase 1 and chtinase 2 are composed of 11 exons and 10 introns. Nevertheless, the whole length of chitinase 2 genomic DNA is much longer than chitinase 1. And in the phylogenetic tree, both of chitinase 1 and chitinase 2 are belong to the acidic chitinase of vertebrate chitinases, but they are in the different branches. Besides, by the analysis of chitinase genes expression in fish larvae, the genes are expressed significantly since 30~33 dpf (day post fertilization). Finally, through the cloning of chitinase genes promoter regions, both of them have the same transcription factors binding sites in the promoter region like the vertebrate pepsinogens. For example, the binding sites of AML-1a, GATA-1, AP-1 and IK-2. To make a summary of this study, the gene expression of chitinase 1 and chitinase 2 have the similar molecular regulatory way like the digestive enzyme in the stomach of vertebrate. In the fish larvae, the gene expression patterns of the two chitinases are similar. But in the phylogenetic tree, chitinase 1 and chitinase 2 are in the different groups. It may drop a hint, even though both chitinase 1 and chitinase 2 are expressed in stomach and have the same function about food digestion, but they may still have some different roles in physiology.
| Identifer | oai:union.ndltd.org:TW/097NCKU5111016 |
| Date | January 2009 |
| Creators | Yen-feng Lin, 林延灃 |
| Contributors | Han-you Lin, 林翰佑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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