碩士 / 國立成功大學 / 環境工程學系碩博士班 / 97 / The effect of ozonation on the cell integrity and metabolite release of Anabaena circinalis and Cylindrospermopsis raciborskii is investigated. The two cyanobacteria were grown in the filtrated water from Tai-Lake, Kinmen, with addition of either ASM or JM algae growth medium. A fluorescence technique, combining fluorescein diacetate (FDA) with either flow cytometer (FCM) and/or epifluorescence microscope (EFM), was used for the determination of cell integrity. A solid-phase microextraction (SPME) concentration followed by a gas chromatograph (GC) and mass spectrometric detector (MSD) was employed to measure the metabolite of A. circinalis, geosmin. A series of ozonation experiments of cyanobacteria-laden water was conducted at different cell concentrations and different ozone dosages. During the experiments, ozone concentration, cell integrity, and metabolite concentration were monitored at different time.
Experimental results indicated that C. raciborskii is very easy to be ruptured by ozone, while A. circinalis is slightly more resistant to ozone oxidation. At 1 mg/L of ozone dosage and 1,500,000 cells/mL of cell concentration, about 30% cells remained integral for A. circinalis after 30 min of reaction, while ~100% cells were ruptured for C. raciborskii. For the metabolites of A. circinalis, geosmin was easily to be destructed by ozone. At ozone dosage of 1 mg/L and cell concentration of 1,500,000 cells/mL, 50% reduction was observed for geosmin after 30 mins of ozonation.
The microphotos from SEM clearly show that the cell surfaces for both strains were damaged after ozonation, with some substances leaked from the cells. This strengthened the observation from EFM and FCM that the cells were ruptured after ozonation. Both strains became fractured at the cell joints.
| Identifer | oai:union.ndltd.org:TW/097NCKU5515016 |
| Date | January 2009 |
| Creators | Li-ling Yeh, 葉俐伶 |
| Contributors | Tsair-Fuh Lin, 林財富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
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