碩士 / 國立東華大學 / 海洋生物科技研究所 / 97 / Stable coral-dinoflagellate endosymbiosis depends on the regulation of nutrition transportation between the symbiont and their host. Symbiodinium sp. resides within vacuoles in the host gastrodermal cells, it translocate up to 60% of photosynthetic products to their coral host, and most metabolites from the Symbiodinium sp. stored as triacylglycerol in the lipid bodies (LBs) within the host cells. In previous study of our lab, we found that LBs presented in gastrodermis were significant larger than epidermis in Euphyllia glabrescens. This suggests that the photosynthetic products from Symbiodinium were mainly stored in gastrodermal LBs. During the bleaching process, the metabolites exported from Symbiodinium sp.to their host were decrease, and the host gastrodermal LBs gradually became smaller and even disappeared. Furthermore, LBs were observed in symbiotic gastrodermal cells (SGCs) in several symbiotic cnidaria, including Aiptasia pulchella (sea anemone), Euphyllia glabrescens, Pocillopora damicornis, Seriatopora hystrix, and tylophora pistillata (coral). As a consequence, the LB was identified as the pivotal organelle for a stable endosymbiosis state.
Little is known about the mechanisms regulate the lipid flux from Symbiodinium sp. into LBs. It was reasonable to assume that the proteins associated with gastrodermal LBs may play a key role in the photosynthetic products translocation. In order to understand the molecular regulation of endosymbiosis, LBs from gastrodermis and epidermis of E. glabrescens were isolated using N-acetylcysteine (NAC), and their protein composition was examined.
In this study, nine groups of LB associated proteins were identified, including lipid metabolic enzymes, struture proteins, chaperones, and membrane traffick associated proteins, etc. These proteins may regulate the nutrition transport during endosymbiosis. Overall, these data indicate that LB is a dynamic organelle and plays a pivotal role in endosymbiosis.
| Identifer | oai:union.ndltd.org:TW/097NDHU5270006 |
| Date | January 2009 |
| Creators | Li-Hsiu Hou, 侯麗秀 |
| Contributors | Chii-Shiarng, 陳啟祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 147 |
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