碩士 / 國立澎湖科技大學 / 海洋創意產業研究所 / 97 / Abstract
Simple methods were established for thallus regenerations from holdfasts, stipes and blades of brown alga Sargassum spp., and determination of suitable medium. Six species of Genus Sargassum were collected at the intertidal zones of Chin-Wan, Wa-Tong , Er-Kan in Penghu from January to October, 2007. The holdfasts, stipes and blades of Sargassum spp. were cultivated with agar media, and the result showed that regenerate rates of holdfasts of S. polycystum and S. hemiphyllum var. chinense could reach 100%. Furthermore, the above two species were cultivated with four different media: agar(086547)、purified agar (A 1296)、high gel strength agar(A9799) and carrageen (C1013) after being immobilized with algin. Regeneration rates of all combinations of treatments and samples also reached 100%. It suggested that holdfasts of Sargassum spp. could be excellent candidates for artificial seeds.
Cultivation of brown alga Sargassum hemiphyllum var. chinense in Penghu in the sea depends on two sources of seedlings: holdfast-derived regenerated seedlings and zygote-derived seedlings. Under natural condition, receptacle formation of the plants began in February, and the eggs were released from March to May. The effective fertilization time of eggs by sperm was found to be within 6~8 hours. However, growth was slightly accelerated if cultured at a temperature of 30℃ compared to other culture temperatures of 15, 20 and 25 ℃. The depth of water had a considerable effect . To explore the optimal condition for growing seedlings on substratum in land-based tanks for avoidance of detachment in this research. The findings of the present study suggest that the cultivation of S. hemiphyllum var. chinense on rope is viable. Furture, the sustainable development of S. hemiphyllum var. chinense farming will rely on a stable supply of artificial seedlings via sexual reproduction under controlled conditions.
| Identifer | oai:union.ndltd.org:TW/097NPHT8833010 |
| Date | January 2009 |
| Creators | Li-Jin Chou, 周立進 |
| Contributors | Jeng-Feng Shyu, 徐振豐 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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