碩士 / 國立屏東科技大學 / 食品科學系所 / 97 / In recent years, the widespread use of chemical pesticides is hazardous effects on the environment and human health. Therefore the organic vegetables are a tendency for healthier foods in the future. The purpose of this study was to screen bacteria that could ferment fish wastes and produced antibacterial components, surveied the best conditions of culture and fermentation to make the environmental safe and biodegradable matters substituting these synthetic insecticides. In this study, a strain producing substrates with high antimicrobial activity was screened from homemade compost. Using fish wastes as fermentation substrate was studied. 10% (v/v) inoculum size inoculated into 10% (w/v) fish wastes adding with 3% (w/v) sugar and 0.1% (w/v) CH3COONa at 30℃ for 15 days with continuous aeration. The supernatant of fermentation broth contained 51 mg/mL of peptide and inhibited the X. campestris and E. carotovora growth obviously with 20 mm and 15 mm clear zone, respectively. In bioassay test, the antimicrobial activity of fermentation broth was influenced accompanying with pH values change. The antimicrobial activity of fermentation broth was not affected after 100℃ heating and ultraviolet and sunlight treatment. Storage temperature didn’t affect the antimicrobial activities of fermentation broth. The C/N ratio was 22. Fermentation broth could inhibit the activity and the leaf consumption of the Pieris rapae crucivora larva. Fermentation broth showed no toxicity and mutagenicity to S. typhimurium TA97 and TA100. Addition of chitosan, tea extract and xian-yuan (food additives) enhanced the antimicrobial activities of fermentation broth. The optimal ratio of fermentation broth to tea extract to chitosan to xian-yuan was 2:1:3:4. The mixture solution showed different antibacterial activities and increased the mortality of Pieris rapae crucivora larva. Field tests, 5% fermentation broth and 1000 folds dilution of the mixture solution could help the Chinese cabbage (pak-cho) growth and increase the index about 220% and 179%, respectively.
| Identifer | oai:union.ndltd.org:TW/097NPUS5253012 |
| Creators | Kuan-Chieh Liu, 劉冠頡 |
| Contributors | Pao-Chuan Hsieh, 謝寶全 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 166 |
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