博士 / 國立屏東科技大學 / 熱帶農業暨國際合作系所 / 97 / The present study aimed to investigate the pathways of stress-induced norepinephrine in modulating both immunity and immune genes expression, and the role of apoptosis in Vibrio alginolyticus infected white shrimp, Litopenaeus vannamei, and to clarify the relationship among stress, immune responses, endocrine modulation and apoptosis. Marked elevations in haemolymph norepinephrine were observed in L. vannamei under hypothermia and hyperthermia in this study. The involvement and stimulatory pathways of norepinephrine on depression of mRNA expression were further examined. In superoxide anion and phagocytotic activity, norepinephrine primarily mediated the depression through both β1 and β2 adrenergic receptors, and however showed no significant difference in phenoloxidase activity and superoxide dismutase activity. The modulations of norepinephrine on immune genes mRNA transcription were further investigated. In prophenoloxidase system, the modulation of NE regulated the prophenoloxidase-I, prophenoloxidase-II, prophenoloxidase-activating enzyme, peroxinectin, and lipopolysaccharide- and β-1,3-glucan-binding protein mRNA transcription through β1, α1, α1 and β1, α1 and β1, and α1 adrenergic receptor-mediated process respectively. In coagulation, NE down-regulated the transcription of tranaglutaminase and clotting protein in haemocyte were through α1, and α1 and β1 adrenergic receptor-mediated process respectively. Depressive effects of NE modulated through various adrenergic receptors on haemocyte in vitro contributed the immunosuppression in L. vannamei. The cysteine aspartate protease-3 (caspase-3) in L. vannamei had been cloned and characterized, and when shrimp injected with V. alginolyticus induced transcription of caspase-3 mRNA and increased caspase-3 activity which contributed to the occurrence of apoptosis and DNA fragmentation in haemocytes. In addition, hypothermia-induced NE plays an important role in the apoptosis of haemocytes in L. vannamei under hypothermal stress, which cause the depressive effects on immunological responses. In conclusion, cold stress induced the release of NE brought haemocyte the depressive effects in immunity and apoptosis respectively, and then caused immunosupression finally.
| Identifer | oai:union.ndltd.org:TW/097NPUS5643010 |
| Date | January 2009 |
| Creators | Chang, Chin-Chyuan, 張欽泉 |
| Contributors | Cheng, Winton, 鄭文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 146 |
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