碩士 / 國立中山大學 / 海洋生物研究所 / 97 / PPAR (peroxisome proliferator activated receptors) is a transcription factor that regulates activity and transcription of enzymes of lipid metabolism and thought PPAR-PPRE (peroxisome proliferator activated receptors – peroxisome prolierator response element) interaction. Cell toxicity was also tested by (3-(4,5-dimethylthiaz ol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay)). The effects of ethyl acetate (EA) extracts of algae and Echinodermata sp. on PPRE-luciferase activity in Huh-7 cell and cell cytotoxicity were conducted. EA extracts of Gracilaria coronopifolia can induce PPRE-luciferase activity without cytotoxity. EA extracts of Pterocladiella capillacea, Gelidium japonicum, and Euchema sp. showed PPRE-luciferase activity and cytotoxicity. Gelidium amansii and Porphyra sp. did not show PPRE-Luciferase activity but had cyotoxicity. EA extracts of Spirulina sp. had PPRE-Luciferase activity but no cytotoxicity, while that of Chlorella sorokiniana inhibited PPRE-Luciferase activity but did not show cytotoxicity. EA extracts of Echinodermata sp. can kill larva and repellent of mosquito, but had no PPRE-Luciferase activity and cytotoxicity. These results demonstrated that the effects of EA extracts on PPRE-luciferase activity are different between algae. EA extract of Gracilaria and Spirulina showed PPRE regulation activity and Echinodermata sp. EA extract have the potential to become green chemical.
| Identifer | oai:union.ndltd.org:TW/097NSYS5270018 |
| Date | January 2009 |
| Creators | Tse-Wei Kuo, 郭澤瑋 |
| Contributors | Tse-Min Lee, 李澤民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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