碩士 / 國立臺灣師範大學 / 生命科學研究所 / 97 / The zebrafish (Danio rerio) represents a powerful model for genetic studies involved in learning and memory function in vertebrates. Scientist developed a variety of genetic mutants by using genetic techniques in zebrafish. While zebrafish mutants are being developed, behavioral researches on learning and memory function in zebrafish are in urgent need. Numerous studies investigated inhibitory avoidance conditioning in other animal model such as rat. However, the study of emotional behavior in teleost fish was still lacking. We used a modified behavioral chamber in our study to study the inhibitory avoidance learning in zebrafish.
Results showed: (1) Twenty-four hour after the training day, all fishes were returned to the shallow compartment again. The escape latency to enter the deeply compartment was significantly longer than pre-training. (2) After exposing to the deeply compartment (extinction training), all fishes were decline in escape latency when compared with the previously test. (3) Seven day after the training day, all fishes were returned to the shallow compartment again. The escape latency to enter the deeply compartment was significantly longer than pre-training. It suggested that this memory can be stored at least seven days. (4) The administration of (+)MK-801(a noncompetitive NMDA receptor antagonist) blocked the expression of inhibitory avoidance behavior in zebrafish. There was a significant difference in escape latency between (+)MK-801-treated group and control group. (5) The phospho-MAPK expression of trained zebrafish’s telencephalon was gradually increasing after training. One and half hour after training, the phospho-MAPK expression of trained zebrafish’s telencephalon reached peak, and there was a significant increase compared with naïve group. The MAPK expression of trained zebrafish’s telencephalon was no significant change.
The present study not only establishes a simple behavior model to study the fear memory in zebrafish, but also suggest the similar involvements of neurochemicals in learning and memory among vertebrates. Thus, it may improve our usage of zebrafish model in neuroscience researches, which may extend to the pace of psychiatric drug discovery, and future studies with zebrafish mutants may be helpful for identify genes involved in learning and memory in vertebrates.
| Identifer | oai:union.ndltd.org:TW/097NTNU5105054 |
| Date | January 2009 |
| Creators | Hsu Chun-Po, 許竣博 |
| Contributors | Lu Kwok-Tung, 呂國棟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 62 |
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