碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / In this study, the grouper iridovirus of Taiwan (TGIV) was propagated in large numbers, and use of PEG (Polyethylene glycol) for virus concentration and sucrose discontinuous gradient for purification. Then the purified TGIV was used as an antigen to immunize hens by intra-muscular injection. Immunized egg yolks were collected, hydrated and spray-dried to prepare egg powders. The ELISA titers of IgY was found higher than 1?32000 in two boosters and the egg powders were higher than 1?64000. The neutralizing antibody titer was 1:640. Furthermore, different concentrations of IgY are trapped by emulsified technique. The encapsulated 10% IgY of oli phase was the best in all groups.The formation rates of the products were 87.75％, and the entrapment rates were 71.5％. The particle size were 22±7.22μm and it could have been preserved under disintegration for more than 1 month at 4℃. After artificial gastric juice processing, the disintegrations was 18.33% after 180 min. Subsequently, encapsulated IgY (including 5%) and non-treated IgY (including 30% and 5%) were mixed individually with eel feed meals to fed grouper .For prevention experiments, TGIV challenge tests were carried out at 1 ,5and 10 days after we finished feeding for 3 consecutive days. The relative survival rates were 50% ,94.5% and 22.2% in encapsulated IgY group, and 40%, 68.8% and 33.3% in non-treated high-dose group, respectively. No protection was found in both non-treated low-dose IgY group and blank egg powder group. For therapeutic experiments, fish in each groups were challenged first with TGIV .After 24 hours and 48 hours, the fish were fed for 3 consecutive days. The group of 24 hours feedings, the relative survival rates was 20% in encapsulated IgY group and 15% in non-treated high-dose group, respectively. No protection was found in both non-treated low-dose IgY group and blank egg powder group. The group of 48 hour feedings, no protection was found in all groups. As a conclusion, the passive protection effect of anti-TGIV IgY has been confirmed and an emulsified technique can improve the efficiency of IgY.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086003 |
| Date | January 2009 |
| Creators | Chia-Ying Chu, 朱家瑩 |
| Contributors | Hsin-Yiu Chou, 周信佑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 95 |
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