碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / Using cyclic voltammetry to measure the reductive potential at different oxygen concentrations, a reductive potential ranging from -0.4 to -0.5v was obtained. The results indicate that the cell density is relative to oxygen consumption, and the residual oxygen in culture medium is proven to be directly proportional to the responding current. We analyzed oxygen consumption of the SB cells infected with TGIV by cyclic voltammetry at -0.4v. When M.O.I. is 1, a continuous increase of the reduction current shows the decrease of the oxygen consumption of cells at 1~4.5 hour post-infection (hpi). The quantity of the oxygen consumption restores to a normal reduction current at 7~10 hpi. At 15 hpi, decrease of the reduction current shows the increase of the oxygen consumption. Further more, SB cell were infected with TGIV at M.O.I.=1、0.1 and 0.01.When M.O.I.=1, the average current was greater than current recorded of M.O.I.=0.1 and 0.01,and the cellular respiration decrease in initial infection. The average current at M.O.I.=0.1 and 0.01 is similar to the result of control. Oppositely, observe cytopathic effect (CPE) was observed until 3~4 days postinfection after TGIV infection. Compared with the traditional CPE observation, amperometric measurement can examine virus infection ealier. Further more, amperometric measurement was used to estimate immunostimulation inhibition for TGIV infection . We detect that sargassum、carrageenan and interferon can suspend the inhibition of total cellular respiration caused dy TGIV infection. This inhibition occured until 12~15 hour post-infection (hpi), indicating that sargassum、carrageenan and interferon can stimulate SB cell secretion antiviral substance.When sargassum and carrageenan concentration at 0.1 mg/ml，and the interferon from crap dilute 50~1000 fold, the titer of TGIV decrease 1~2 log TCID50/ml. MTT assay results indicate that there is no obvious cellular response variation after immuostimulant connect with SB cell. We suggest that immunostimulant would not increase cellular response，but inhibit virus infection directly.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086004 |
| Date | January 2009 |
| Creators | Chia-Wei Chang, 張家緯 |
| Contributors | Hsin-Yiu Chou, 周信佑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 76 |
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