碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / In euryhaline teleosts, gills play an important role in osmoregulation and ionoregulation, but also have an immunization and stress response function. The objectives of the present study were to investigate the expression and possible roles of CRH, CRHR1, HSP70, IL-1β, Na+/K+ATPase α and PRLR genes in the gill and brain of black porgy (Acanthopagrus schlegeli) in response to stress.
We conducted salinity stress experiments by transferring the fish to freshwater (FW) from seawater (SW) and also CRH and Dexamethasone and the changes in respective gene expressions were analyzed in the gill and brain by quantitative real-time PCR measurements. Transfer of fish from SW to FW resulted in an increased expression of HSP70 and Na+/K+ATPase α mRNA expression in brain on day 7. In contrast, CRHR1,HSP70, IL-1β, Na+/K+ATPase α and PRLR mRNA expressions in the gill exhibited a significant increase on both day1 and day 7.Injection of CRH resulted in increased CRH expression in both brain and gill. On the other hand, injection of Dexamethasone resulted in an increase in CRH gene in the forebrain and pituitary, while CRHR1 gene decreased in forebrain.
Immunohistochemical and in situ hybridization studies were also conducted to identify Na+/K+ATPase α, crh and crhr1 localization in gill. The results suggest important roles for gill in during stress.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086022 |
| Date | January 2009 |
| Creators | Tsan-Ping Wang, 王贊評 |
| Contributors | Ching-Fong Chang, 張清風 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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