碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / The objectives of this study were to investigate the heat shock protein 70（Hsp70）and Gonadotropin-releasing hormone （GnRH）in scleractinian coral, Euphyllia ancora. The possible roles of Hsp70 and GnRH in the gonad development and maturation of coral were also studied. By applying “cDNA library construct” were cloned two heat shock protein 70-like genes fragment（hsp70-like） in Euphyllia ancora, and temporarily to named hsp70a and hsp70b. Heat shock protein 70（Hsp70）was stress and gametogenesis-related gene in vetebrate and invertebrate, but the possible roles of heat shock protein 70 in coral were not clear. The objectives of were to investigate the expression and relationship of Hsp70 in the gonad development and maturation of coral. In the study, hsp70a in female and male had no significantly change from non-spawning season to spawning season, but female presented higher expression of hsp70a than male. In male corals the expression of hsp70b decreased from non-spawning season to spawning season, but in female corals the expression of hsp70b increased from non-spawning season to spawning season. It’s relationship with oogenesis. Another the study by the cultured of gonad cell to track to metabolic products of 「3H」Pregnenolone during gonad maturation, and the objectives of were to investigate the possbible roles of GnRH in the gonad development and maturation of coral. In the study, we found the sex steroid hormone metabolic products of 11KT、20βS、S、E2、AD、DOC＆T、17OHP、DHP、P5 , and in male gonad tissue the concentration of 11-ketotestosterone increased by LHRH-A treated. In female ovary the concentration of Estradiol 、 Estrone和Androstenedione increased by LHRH-A treated. GnRH had the directly regulative function to the coral gonad, and extrapolated the coral should have the completely mechanism of sex steroidogenesis.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086027 |
| Date | January 2009 |
| Creators | Huo-Jui Hsieh, 謝和叡 |
| Contributors | Ching-Fong Chang, 張清風 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
Page generated in 0.002 seconds