碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / The purpose of this study was to investigate the vitamin B6 24hr metabolism in hybrid tilapia the two input methods (forced feeding and injection) with a modified cannulated urine collector in order to monitor the changes of plasma and urine vitamin B6 concentration within after the intiation of certain amount of exogruous vitamin B6 supply lithor by oral administration or by injection a 24-hour duration .
There were three replicates each having 3 runs of individual fish at a time, every fish was randomly received either an injection of 5ml vitamin B6 solution at a certain concentration of 0, 0.1, 0.3, 0.5, 1 ppm, or a vitamin B6 containing diet at a certain level of 0, 5, 10, 20, 40, 80ppm, in concentration .
It was observed that plasma vitamin B6 concentration in the fish of the injection group were peaked 2-4 hours earlier than those fish in the oral administration group, however the urine B6 concentration umory the all treatments writhin each of the two groups of fish were all peaked at the 4-6th hour. There were no significant differences in the liver and muscle vitamin B6 concentration among the treatments either in the injection group or in the oral administration group of fish. The range was between 240-250ppb in the muscle, 2400-2410ppb in the liver for the oral administration group of fish, while that for the injection group was 230-240ppb in the muscle and 2440ppb in the liver.
The delay of the peak of the plasma and urine B6 concentration in the oral administration group revealed that vitamin B6 must have been absorped slowly in the digestive system at least four hours before transported to circulation system even given a high level of supply either by injection or oral administration.
This finding indicated that the vitamin B6 excrated rate did not increase in proportion to the iucreasing level vitamin B6 supply though the absolute amount suggested that there B6 excretion did by the level enzyme available rathes than substrate level B6.within the 24-hr duration of B6 excretion in hybrid tilapia was limited. The value of the regression line slope of the urine B6 concentration against plasma B6 concentration was observed higher in the fish with orally administrated 10ppm B6 diet than those fish orally received other concentrations of vitamin B6 diets, and higher in the fish injected a dose of 0.3ppm B6 solution compare with those fish injected different doses. It was concluded that the most suitable level in B6 utilization was 10ppm for oral administration and 0.3ppm for injection.
Adding an indication such as innulin in vitamin B6 solutions for a clearance test as well as extending the experimental duration from 24 hours were recommended to assure more detailed and correct data could be collected.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086050 |
| Date | January 2009 |
| Creators | Tzu-Yang Line, 連子揚 |
| Contributors | Yii-Shing Huang, 黃沂訓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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