碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / The objective of this study was to investigate the vitaminB1 metabolism of hybrid tilapia (Oreochromis niloticus × O.aureus) with a modified cannulated urine collector through the comparisons between two exogenous vitaminB1 supply methods, injection and oral administration, within a 24 -hour duration.
There were three replicates each running three fish at a time. Each fish received an exogenous supply of either an injection of 5ml vitamin B1 solution with a certain concentration of 0, 0.1, 0.3, 0.5 and 1ppm, or an oral administration of 0.3%BW vitamin B1 diet at a certain level of 0, 1, 4, 8, 12 and 16ppm in concertration. Blood and urine samples were taken at the 2nd, 4th, 6th, 8th, 10th, 12th, 16th, 20th and 24th hour after the initiation of each run of the experiment. Muscle and liver samples were obtained at the 24th hour in the end of the experiment.
It was observed that the plasma concentrations of the whole treatments were all peaked at the 6th hour and urine concentration peaked at the 6th-8th hour after oral administrations of diets. The plasma vitamin B1 peaked at the 2nd hours ,while that for vitamin B1 was at the 4th hour after injection. The delay of 4-6 hours of the plasma vitamin B1 concentration peak and the delay of 2-4 hours of the urine vitamin B1 concentration peak in the oral administration group of fish than the respective two peaks in the injection fish group revealed that vitamin B1 had been absorped in the digestion system 4-6 hours before enough amount of vitamin B1 was transported to circulation system.
The amount of vitamin B1 excreted in urine within the 24-hourr experimental duration were 1.60×10-2μg for fish given control diet, 1.22×10-1μg for fish given 1ppm vitamin B1 diet, 2.02×10-1μg given 4ppm diet, 3.20×10-1μg given 8ppm diet, 3.80×10-1μg given 12ppm diet and 4.57μg given 16ppm diet, respectively. While the respective vitamin B1 concentration in urine within the 24-hour experimental duration amounted to a total of 1.62×10-2μg for fish injected control solution, 4.74×10-2μg for fish injected 0.1ppm vitamin B1 solution, 1.15×10-1μg for 0.3ppm solution, 2.43×10-1μg for 0.5ppm solution and 4.85×10-1μg for 1ppm solution. But the amount of vitamin B1 excreted in the urine did not match the amount in proportion in blood which led to a low accumulated percentage excretion for the whole treatments in both injection or oral administration group of fish. By plotting the values of urine vitamin B1 concentration against those of plasma vitamin B1 concentration , it was found that oral administration of 1ppm vitamin B1 diet group of fish and the injection of 0.3 ppm solution group of fish exhibited a higher value of the regression line slope than those of the other treatments compared each in the two respective vitamin B1 supplying methods. So it was concluded that 1ppm diet and 0.3ppm injection of vitamin B1 must be the candidate level for best utilization in hybrid tilapia.
Adding an indicator such as innulin in vitamin B1 solutions for a clearance test as well as extending the experimental duration from 24 hours to 48 hours were recommended to assure more detailed information about the vitamin B1 metabolism of this fish be collected.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086051 |
| Date | January 2009 |
| Creators | Lin Yuan-Cheng, 林園程 |
| Contributors | Yii-Shing Huang, 黃沂訓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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