碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 97 / In recent years, shrimp diseases have caused catastrophic economic losses in the shrimp aquaculture industry throughout the world. By elucidate the innate immunity may play an important role in protecting shrimp from pathogen infection it may also shed light on developing strategies to save the shrimp aquaculture industry. The objective of present study is to study immune responds in penaeid shrimps by DNA microarray and transcriptomics. In present study, Penaeus monodom and Litopenaeus vannamei were used as experimental animals.
To fulfilled the objective, several specific aims was established, 1) to establish DNA chip related to tiger shrimp immune genes and study the expression profile of high disease resistance tiger shrimp; 2) to establish platform technique for hatchery practice using candidate gene markers to screen disease resistant shrimp broodstocks; 3) to study the immune response in tiger shrimp followed various microbial-immune-elicitors. (MIE treatment) ; 4) to employ bioinformatics to establish white shrimp immune-related oligo-DNA microarray; 5) to study the immune response in white shrimp by administering various MIEs and Chinese herbs compounds; 6) to apply nutrigenomics concept to establish platform technique to screen various immunestimulants as aquafeed feed additives. In present study, thirty immune-related genes were isolated and generated a shrimp immune DNA microarray, the result indicated that higher mRNA level of hemocyanin and 5HT1-receptor was found in high disease-resistant shrimp broodstocks. The expression level of crustin, penaeidin, haemocyanin and dicer genes showed higher after injection with poly I:C at 48 and 72 hours. This suggested that poly I:C can be used to screen higher disease resistant shrimp in shrimp hatchery of practice operation. The application of shrimp immune DNA microarray in nutrigenomic study revealed that the expression levels of immune genes, such as, crustin, lysozyme, mo-penaeidin, transglutaminase and kazal-type proteinase inhibitor increased significantly respectively after injection of lipopolysaccharide, peptidoglycan or β-glucan in tiger shrimp. Similar result was also found that the transcripts level of immune genes (crustin, lysozyme, Mo-penaeidin, transglutaminase and kazal-type proteinase inhibitor) could be induced and the transcript level increased significantly in white shrimp after injecting or feeding with immunestimulants (such as Chinese medicine herbs and various MIE). The subsequent results of pathogens challenge test demonstrated that the survival rate of fed with Chinese medicine herbs group were improved 13﹪and 28﹪better than non-fed Chinese medicine herbs controls using Vibrio Harvey or WSSV to infect white shrimp,.
In present study, a variety of immunestimulants can effectively enhance immunity and disease resistance of P. monodon and L.vannamei. The application in microarray as a experimental platform and use the transcriptomics to understand the regulation of gene expression, in screening of high disease resistance shrimp and screening of high-performance feed additive nutrition. The DNA microarray certainly helps marker-assisted selection (MAS) and nutrigenomic application.
| Identifer | oai:union.ndltd.org:TW/097NTOU5086052 |
| Date | January 2009 |
| Creators | Yi-Ting Hsiao, 蕭宜庭 |
| Contributors | Jenn-Kan Lu, 陸振岡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 133 |
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