碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / The inhibitory effect of Chlorella sorokiniana hydrolysates (hydrolyzed by cellulase and protease) on activity of α-glucosidase and α-amylase were investigated. When adding of 5% cellulase and 2% protease in the three Chlorella hydrolysates (pulverized cell, unpulverized cell and residue), the hydrolysate (pulverized cell) showed the highest yield being 42.0% after hydrolysis for 3 h. As the hydrolysates (pulverized cell) were hydrolyzed for 5 h, the peptide and free amino acid content of Chlorella hydrolysates (pulverized cell) were increased from 236.6 to 304.0mg/g and from 34.4 to 61.1mg/g, respectively. As hydrolysis for 3 h, the hydrolysate had the highest inhibition on α-glucosidase (IC50＝26.4 mg/mL) and α-amylase (IC50＝20.7 mg/mL). And, total polyphenol content (24.8mg/g) reached the highest value. The hydrolysate (pulverized cell) was separated into four fractions by size exclusion chromatography on a Sephadex-15 column. The fourth fraction (M.W.=370-280Da) of the hydrolysate showed the highest α-glucosidase inhibitory efficiency ratio (IER) in carbohydrate, peptide and polyphenol compound as base, which were 126.4, 122.0 and 316.1 %/mg/mL. The fourth fraction was further analyzed by HPLC, only peak Ⅳ-1 had inhibitory effect on α-glucosidase and its IER was 1027.8 %/mg/mL.
| Identifer | oai:union.ndltd.org:TW/097NTOU5253011 |
| Date | January 2009 |
| Creators | Ying Wu, 巫熒 |
| Contributors | Jenn-Shou Tsai, 蔡震壽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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