碩士 / 國立臺灣海洋大學 / 食品科學系 / 97 / In the present study, two methods, chlorsulfonic acid-pyridine (PRI) method and chlorsulfonic acid-N,N-dimethylformamide (DMF) method, were used to increass sulfate content of M. nitidum oligosaccharides (MnOS). And the antioxidant and anticoagulants activities of there various sulfate contents MnOS were compared. The sulfate contents of SMnOS1-3kDa-PRI (1-3 kDa MnOS modified by PRI method), SMnOS3-5kDa-PRI (3-5 kDa MnOS modified by PRI method), SMnOS1-3kDa-DMF (1-3 kDa MnOS modified by DMF method), and SmnOS3-5kDa-PRI (3-5 kDa MnOS modified by DMF method) were 2.24% to 8.58%, 2.80 to 8.58%, 2.24% to 2.24% 4.18%, and 2.80% to 4.77%, respectively. As to the ability of chelating on Fe (II) ion of MnOS, the results indicated that MnOS1-3kDa and MnOS3-5kDa were 68.02% and 66.23%, and SMnOSs were not detected. DPPH free radicalscavenging effect of MnOS and SMnOS were not detected. The structures of the SMnOSs derivatives were analyzed by FT-IR spectroscopy, the photograms showed that the sulfate groups of MnOS and SMnOS. MnOS had specific absorption bands of R-SO2-R groups in the area of 1100-1200 cm-1. SMnOS1-3kDa-PRI and SMnOS3-5kDa-PRI had specific absorption bands of 1100-1200 cm-1 and 1409 cm-1, which representing fluctuations of R-O-SO2-R or R-O-SO2-O-R, and the absorption peaks of C=O fluctuation at 1680 cm-1. SMnOS1-3kDa-DMF present absorption peaks of R-O-SO2-O-R and R-SO-R in the area of 1469 cm-1 and 1022 cm-1, SMnOS3-5kDa-DMF as well as there are bands of R-NH2 bonds in the area of 1613 cm-1, and also fluctuations of R-SO2-R group in the area of 1613 cm-1. The APTT of anticoagulants activity test was showed SMnOS1-3kDa-PRI could decreased 39.80% coagulation edffect than MnOS1-3kDa did, and SMnOS3-5kDa-PRI could decreased 27.14% coagulation edffect than MnOS3-5kDa had. SMnOS1-3kDa-DMF could decreased 21.14% coagulation edffect than MnOS1-3kDa, and SMnOS3-5kDa-DMF could decreased 21.78% coagulation edffect than MnOS3-5kDa. The result of PT of anticoagulants activity test was similar on MnOSs and SMnOSs. Totally, the results indicated that chlorsulfonic acid-pyridine method and chlorsulfonic acid-N,N-dimethylformamide method can enhance sulfate content to 8.58% and 4.77%, and SMnOS can decrease the optical density in APTT test (39.80% and 27.80% ) at 0.5 mg/mL.
| Identifer | oai:union.ndltd.org:TW/097NTOU5253069 |
| Creators | Chaio Li, 李樵 |
| Contributors | Chorng-Liang Pan, Ph.D., 潘崇良 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 124 |
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