碩士 / 國立臺東大學 / 生命科學研究所 / 98 / Abstract
Antrodia camphorate is a Taiwan-specific medical fungus, and its fruit-body is
often applied to the treatment of various diseases such as sitotoxism and drug toxicity
induced diarrhea and abdominalgia, hypertension, pruritus and cancer. Amyloid
β-peptide (Aβ) is proven to closely associate with pathogens of Alzheimer’s diseases
(AD). The deposition of Aβ in brain causes oxidative stresses and inflammatory
responses, and leads to neuron damages as well as the impairments of learning and
memory. Therefore, antioxidant capabilities are highly associated with AD
preventions. A number of animal experiments have indicated that A. camphorata
would provide excellent in vivo antioxidant effect, so antioxidant effect is probable to
be used as the important marker for AD prevention. This study concerned in the
research and development of an A. camphorata fermentation product with the
functions of prophylaxis and amelioration of AD. AD prevention factors of
A.camphorata fruit-body and mycelium including DPPH scavenging effect, anti-Aβ
neurocytotoxicity, antioxidative and anti-inflammatory capacities were measured in
this study. AD rat model established through continuous injection of Aβ40 for 28
consecutive days was used to evaluate the effect of A. camphorata fermentation
products on learning and memory abilities, as well as oxidative and inflammatory risk
factors in cerebral cortex and hippocampus tissues. The results showed that the water
extract of fruit-body had the most potent DPPH scavenging ability among all groups.
The ability for the protection against Aβ40 cytotoxicity were the water extract of
mycelium, lovastatin, the water extract of fruit-body, the ethanol extract of fruit body
and the ethanol extract of mycelium in sequence expressed as using the concentration
at 1.25 ppm. The ability for the protection against Aβ25-35 cytotoxicity were the
water extract of fruit-body, the water extract of mycelium, the ethanol extract of
fruit-body, the ethanol extract mycelium, and lovastatin in sequence expressed as
using the concentration at 1.25 ppm. In the cell test regarding the prevention against
Aβ-induced oxidative stress and inflammatory response, malondialdehyde (MDA) and reactive oxygen species (ROS) expression were both significantly increased by
the treatment of Aβ40 (187.5 nM) and Aβ25-35 (10 μM) in PC 12 cell (p<0.05).
However, these damages were be reversed by the treatment of water and ethanol
extract of fruit body and mycelium, in which the water extract of fruit body performed
the best effect. The Tau protein in PC 12 cells was expressed by the treatment of Aβ40,
as compared with Normal group. However, this expression was supressed by the
water extract of fruit body. Furthermore, the results of the experiments concerning
animal learning and memory ability showed that AD rats fed fruit-body and mycelium
have shorter searching time in the reference memory experiments and working
memory experiments (p<0.05). In addition, the fruit-body group would extend the
searching time around target quadrants in the spatial exploration tests (p<0.05). The
concentration of MDA and ROS in cerebral cortex and hippocampus tissues of Aβ
group were higher by 2, 7.1 and 2 times than that of the Normal group (p<0.05), but
the increased MDA and ROS levels may be effectively brought down by feeding
fruit-body and mycelium (p<0.05). According to the result of the Aβ40 and sAPPα
protein deposition in cerebral cortex and hippocampus tissues, a large amount of
Aβ40 deposit is discovered in Aβ group, as compared with the Normal group. An
obvious decreasing of Aβ40 deposition was shown in fruit-body group. According to
the expression of Aβ40, BACE, and Tau protein in hippocapus and cortex tissue,
Aβ40, BACE, and Tau were significantly expressed in Aβ-infused rat. However, the
fruit body treatment was able to perform more effect on reversing the damage than
mycelium treatment . Lower sAPPα deposition was also generated in cortex tissues in
the Aβ group than in Normal group, but a remarkable increase of sAPPα accumulation
is further observed in the lovastatin group and fruit-body group as compared with the
Aβ group. In the future work, the result of this study was expected to be the base on
the study of the functional ingredients and mechanism of AD prevention of A.
camphorate.
| Identifer | oai:union.ndltd.org:TW/097NTTU0105001 |
| Date | January 2010 |
| Creators | Shen-En Wang, 王聖恩 |
| Contributors | Chun-Lin Lee, Ph.D., 李俊霖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 119 |
Page generated in 0.009 seconds