碩士 / 國立臺灣大學 / 動物學研究所 / 97 / Cofilins are members of actin-depolymerizing factor (ADF)/cofilin protein family that regulate actin treadmilling and the extension of pseudopods in motile cells. To study cofilin functions in gastrulation cell migration, we have isolated cofilin 1 (cfl1) from zebrafish genome, and its expression and functions have been examined. By RT-PCR and whole-mount in situ hybridization, cfl1 has been found to be ubiquitously expressed during early embryogenesis. We have also demonstrated that knockdown of cfl1 interfered cellular migration during epiboly, convergence and extension. Furthermore, the effects of cfl1 MO was found to be cell autonomous by transplantation experiments. In addition, pseudopods in the involuting cells were significantly reduced in the cfl1 morphants. The effects of cfl1 MO was further evident by the loose cell adhesions between enveloping layer (EVL) and deep cell layer (DEL) through transmission electronic microscopy and confocal microscopy. These results suggest that cfl1 participates in epiboly, involution, convergence and extension during zebrafish gastrulation.
| Identifer | oai:union.ndltd.org:TW/097NTU05312004 |
| Creators | Chun-Wei Lin, 林浚緯 |
| Contributors | Shyh-Jye Lee, 李士傑老師 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 37 |
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