碩士 / 國立臺灣大學 / 漁業科學研究所 / 97 / Tilapia is one of the main cultured fish in the world and the aquacultured production in 2007 was about 2,500,000 tons. Therefore, the demand of feed in tilapia aquaculture is enormous. In General, tilapia could use plant protein sources and carbohydrate efficiently. So the lipid sources and its efficiency may cause important effect for cost of feed. In present study, red tilapia diets were supplemented with additional 5% lipid from different proportion (0%, 2%, 3% or 5%) of either squid oil, beef tallow or soybean oil to investigate the influence of growth performance, body fatty acid composition and essential fatty acid demand under different temperarure.
In experiment one, nine diets were supplemented with additional 5% lipid from different proportion (0%, 2%, 3% or 5%) of either squid oil, beef tallow, soybean oil and one diet was not supplemented with any additional lipid (Ctrl). Red tilapia fingerlings (1.21±0.04g) were fed in average 22±2℃ condition for ten weeks. After feeding trial, growth performance was significantly higher (p＜0.05) in red tilapia fed with S5 (squid oil 5%) than in fish fed SB2S3 (soybean oil: squid oil=2:3) and Ctrl. Other groups were not significantly different (p＞0.05) compared with each other. Fatty acid composition in whole body and non-polar lipid fraction from liver reflected the composition of diet. And red tilapia fed with S5, T5 (beef tallow 5%), T2SB3 (beef tallow: soybean oil=2:3) and SB3S2 (soybean oil: squid oil=3:2) were satisfied n-6 series and n-3 series fatty acid requirement by calculating the ratio of 20:3n-9/20:4n-6 and 20:3n-9/22:6n-3 in polar fraction from liver.
In experiment two, three diets were supplemented with additional 5% lipid from either squid oil, beef tallow and soybean oil and one diet was supplemented with 3% soybean oil and 2% squid oil. Red tilapia fingerlings (0.74±0.03g) were fed in 20℃ (low temperature, LT) and 27℃ ( high temperature, HT) condition for eight weeks. After feeding trial, growth performance was significantly higher (p＜0.05) in HT groups than in LT groups. In HT groups, growth was significantly higher (p＜0.05) in T5 than in S5 and other treatments were not significantly different (p＞0.05) compared with each other. In LT groups, growth was significantly higher (p＜0.05) in SB5 (soybean oil 5%) than in S5 and SB3S2 but other treatments were not significantly different (p＞0.05) compared with each other. Fatty acid composition in whole body and non-polar lipid fraction from liver reflected the composition of diet. Moreover, red tilapia fed with SB3S2 was not satisfied n-6 series fatty acid requirement both in LT and HT conditions.
| Identifer | oai:union.ndltd.org:TW/097NTU05451020 |
| Date | January 2009 |
| Creators | Han-Sheng Wang, 王漢昇 |
| Contributors | 廖文亮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
Page generated in 0.0087 seconds