碩士 / 國立臺灣大學 / 獸醫學研究所 / 97 / The fish aquaculture was rapid development in Taiwan and the high density caused the highly risks for suffering several diseases. In order to treatment the diseases of fish, not only improving the cultivating environments and techniques, but also tried various chemical therapy drugs widely. Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for use in veterinary medicine. Copper sulfate and potassium permanganate were enabled to use for keeping away the harmful organism as surface parasite, bacteria, alga and other harmful living organisms in water. The danofloxacin, copper sulfate and potassium permanganate were non- legal aquaculture drugs for fishery at present in Taiwan, but in fact the drugs were used commonly in aqua-cultured field and they used them mostly consult the foreign literature report. The purpose of this research provided the residues and acute toxicity of danofloxacin, copper sulfate and potassium permanganate individually to tilapia and grouper for fishery references.
After single oral administration (10 mg/kg b.w.), the plasma, kidney, liver and muscle were collected, then the danofloxacin concentration of samples was determined by using high performance liquid chromatography. In tilapia, the result indicated that the maximal plasma, kidney and liver concentration were 1.438 μg/mL, 13.178 μg/g and 12.477 μg/g, respectively, at 6 h after administration. The maximal muscle concentration was 2.153 μg/g at 12 h after administration. The elimination half-time of plasma, muscle, kidney and liver were 28.76 h, 33.48 h, 43.58 h and 48.46 h, respectively. In grouper, the result indicated that the maximal plasma, kidney and liver concentration were 2.781 μg/mL, 5.020 μg/g and 0.753 μg/g, respectively, at 1 h after administration. The maximal muscle concentration was 0.450 μg/g at 4 h after administration. The elimination half-time of plasma, muscle, kidney and liver were 47.14 h, 28.06 h, 32.08 h and 41.50 h, respectively. The ED50 of danofloxacin in tilapia and grouper were respectively determined after examined by histopathological section. The ED50 of danofloxacin in tilapia were 37.5 ppm and 81.67 ppm, after administration for 3 days and 7days, respectively. The ED50 of danofloxacin in grouper were 173.33 ppm and 94.33 ppm, after administration for 3 days and 7days, respectively.
By utilizing the atomic absorption spectrophotometer to determine the fish''s body residues of amount of copper and manganese. After 24h drug bath of copper sulfate and then transferred to clean water for 10 days, the residues of copper in muscle and liver of tilapia were 0.2087 ppm and 2.5373 ppm, respectively. On the other hand, the residues of copper in muscle and liver of grouper were 0.1769 ppm and 2.4583 ppm, respectively. After 4h drug bath of potassium permanganate and then transferred to clean water for 10 days, the residues of manganese in muscle and liver of tilapia were 0.2836 ppm and 1.9741 ppm, respectively. On the contrary, the residues of manganese in muscle and liver of grouper were 0.2024 ppm and 1.7524 ppm, respectively. The 96h LC50 of copper sulfate in tilapia and grouper were 30.5 ppm and 1.36 ppm, respectively. The 96h LC50 of potassium permanganate in tilapia and grouper were 3.60 ppm and 1.42 ppm, respectively.
| Identifer | oai:union.ndltd.org:TW/097NTU05541005 |
| Date | January 2008 |
| Creators | Yang-Chi Fan, 范揚棋 |
| Contributors | 郭宗甫 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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