博士 / 國立臺灣科技大學 / 高分子系 / 97 / Two blend hydrogels, glucan/poly (vinyl alcohol) and sodium alginate/calcium poly(γ-glutamate)were prepared. The properties of the resulting hydrogels were evaluated through hydrophilicity tests, mechanical tests, and biocompatibility. This research was divided into two parts.
In the first part, aqueous mixture of β-glucan and poly(vinyl alcohol) (PVA) was cast into films and dried at 110°C without chemical crosslinking. The content of glucan in the film varied from 7% to 50%. The hydrophilicity of the resulting films was evaluated with swelling tests, wet area diffusion tests, and water vapor transmission tests. The swelling ratio, the wetting ratio, and the water vapor transmission rate increased with the glucan content. When contacting water, glucan was released, and the percent release of glucan increased with the glucan content. The addition of glucan made the film more ductile than pure PVA. The results of hemocompatibility test showed no significant effect on the activated partial thromboplastin time (APTT) and thrombin time (TT) and minor adsorption of human serum albumin (HSA). On observing the wound healing of rat skin, the healing time was shortened by 48% using glucan/PVA film comparing to cotton gauze. Therefore, a wound dressing made of glucan/PVA can greatly accelerate the healing without causing irritation.
In the second part, sodium alginate (Alg) hydrogel films were crosslinked with either calcium poly(γ-glutamate) (Ca-PGA) or CaCl2. The hydrophilicity of the resulting hydrogel films was evaluated through swelling tests, water retention capacity tests, and water vapor permeation tests. The swelling ratio, water retention capacity, and the water vapor transmission rate of Alg/Ca-PGA (AG) were higher than those of Ca-Alg(AC). The swelling ratio of Alg/Ca-PGA was 651% and 190% at pH 7.4 and pH 1.2, respectively. The tensile strength of Alg/Ca-PGA hydrogel was lower than that of Ca-Alg. The results of hemocompatibility test showed that Alg/Ca-PGA caused shorter activated partial thromboplastin time (APTT) than Ca-Alg. Both Ca-Alg and Alg/Ca-PGA exhibited almost no adsorption of human serum albumin (HSA), whereas the adsorption of human plasma fibrinogen (HPF) of Ca-Alg was 10 times of that of Alg/Ca-PGA. In addition, Alg/Ca-PGA exhibited platelet adhesion higher than Ca-Alg. Furthermore, both Alg/Ca-PGA and Ca-Alg exhibited no cytotoxicity.
| Identifer | oai:union.ndltd.org:TW/097NTUS5310045 |
| Date | January 2009 |
| Creators | Mei-hua Huang, 黃美華 |
| Contributors | Ming-chien Yang, 楊銘乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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