碩士 / 靜宜大學 / 食品營養研究所 / 97 / Chlorella is a unicellular green microalgae that contains multiple nutrients. Previous studies have demonstrated that chlorella shows antioxidant, antihypertensive, antilipidemic, anti-inflammatory and antitumor effects. The purpose of this study was to investigate the effects of chlorella extracts on learning and memory ability, and antioxidant status in senescence-accelerated mice (SAMP8). Three and six-month-old SAMP8 male and female mice were used in this study. SAMP8 mice were randomly divided into four groups, where each group contained 15 mice, including control group, low dosage-0.14ml, medium dosage-0.27ml and high dosage-0.81ml group. This study period lasted for 12 weeks. Besides food intake, the body weight was also measured. One week before scarification, the aging scores were measured, open field activity test was recorded in evaluation for behavior, and single-trail passive avoidance test and active shuttle avoidance test were performed independently. The mice were sacrificed to analyze the biochemical parameters of sera. The antioxidant ability of liver, the lipid peroxidation of liver and brain, the liver contents of protein carbonyl and total thiol andβ-amyloid protein deposition of brain were measured. The results showed that there was no significant difference in the body weight, food intake, and locomotion among each group. The aging score of control groups was significantly higher than experimental groups in 3 and 6-month old mice (p＜0.05). Results from ingle-trail passive avoidance test and active shuttle avoidance test showed that experimental groups had significantly better learning memory ability (p＜0.05). Activities of antioxidant ability, including SOD, GPx, catalase, and total thiol , in the experimental groups were significantly higher than that of control groups (p＜0.05). The MDA of liver and brain, and hepatic protein carbonyl liver in the experimental groups were significantly lower than the control groups (p＜0.05). Besides, theβ-amyloid protein deposition of brain in 6-month male and female mice were significantly lower in the experimental groups than control groups (p＜0.05). In summary, the supplement of chlorella extract may promote the antioxidant defense system, reduce oxidative stress-induced damage, reduce the accumulation ofβ-amyloid deposition, and improve learning and memory ability. The underlying anti-aging mechanism needs further investigation.
| Identifer | oai:union.ndltd.org:TW/097PU005255018 |
| Date | January 2009 |
| Creators | Ya-Ching Hsu, 許雅淨 |
| Contributors | Ming-fu Wang, 王銘富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 133 |
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