碩士 / 大仁科技大學 / 環境管理研究所 / 97 / In order to convert CO2 in flue gas from the coal-fired thermal power plant into biomass by solar energy, we conducted a study to define growth characteristics of Spirulina maxima under various culture conditions. In this study, S. maxima was incubated in an outdoor pond with open raceways. In order to evaluate and compare both the CO2 fixation efficiency and the cultivating performance of S. maxima under different CO2 and flow velocity conditions, five different setup options were carried out, namely (Blank) 0.035% CO2 with 15 cm s-1, ( I ) 6% CO2 with 15 cm s-1, (Ⅱ) 6% CO2 with 30 cm s-1, (Ⅲ) 3% CO2 with 15 cm s-1 followed by 0.035% CO¬2 with 15 cm s-1 and (Ⅳ) 3% CO2, 30 cm s-1 followed by 0.035% CO2 with 30 cm s-1. The characterization of organic matters released from algal water was defined by Excitation/Emission Fluorescent Matrix (EEFM). Furthermore, the cell composition of S. maxima was compared through the analysis of carbon content, carbohydrates, pigments and amino acids.
The result indicated that the maximum cell biomass production of 0.908 g (dry-weight) L-1 was obtained under the runⅡconditions of 6% CO2 with 30 cm s-1. The productivity value of 9.1 g (dry-weight) m-2 d-1 and the CO2 fixation value of 15.83 g CO2 m-2 d-1 were both found to be the highest under the runⅡsetup as well.Furthermore, a delivered CO2 cost of 50 (TWD) kg-1 and a production cost of 87 (TWD) for per kg dry S. maxima were estimated under the runⅡoption. The annual delivery amount of CO2 from flue gas into microalgae pond was estimated to be about 51.53 ton with an annual biomass production of 29.49 ton dry-weight S. maxima.
The EEFM plots of S. maxima show that two peaks occurring at wavelengths lower both than 400 and 640 nm, indicating which are related to protein-like and pigment, respectively. The algal water, on the other hand, occurs two peaks at wavelengths lower both than 400 and 440 nm, indicating which are related to protein-like and humic-like organics, respectively. Moreover, the ratios of the emission intensity at a wavelength of 450 nm to that at 500 nm, obtained in our study with an excitation of 370 nm, are all larger than 1.9. Moreover, the increase of NPDOC (Non-Purgeable Dissolved Organic Carbon) in algal water and carbon content in dry biomass were higher in run Ⅲ and Ⅳ(3% CO2 ) than those in run I and II (6% CO2).
The carbohydrate content of S. maxima in Blank run was found to be higher than that in any other runs. β-Carotene dominates the pigment content of S. maxima with Chlorophyll a as the second highest content. Furthermore, Argentine, Glutamine and Aspartic acid, together contribute 40% of total amino acid content, are the three major amino acids in S. maxima.
| Identifer | oai:union.ndltd.org:TW/097TAJ05700002 |
| Date | January 2009 |
| Creators | Kun-Hsin Lin, 林坤信 |
| Contributors | none, none, 李崇垓, 賴文亮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 151 |
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