碩士 / 淡江大學 / 生命科學研究所碩士班 / 97 / capsulin and musculin are two related transcription factors that controlled cranial muscles development in vertebrate, but their functions still unclear. Here, we used zebrafish as an animal model to study their roles during early developmental stage. After sequence comparison, we found that the zebrafish capsulin (musculin) polypeptide shares sequence identities of 75%(63%), 75%(63%), 76%(63%), 75%(63%) and 71%(62%) with the reported capsulin (musculin) of human, bovine, mice, mouse and Xenopus, respectively. Whole mount in situ hybridization experiments showed that capsulin was first detected at mesoderm of 20 hours post-fertilization (hpf) embryos. Comparing with expression pattern of dlx2a, we found that capsulin was expressed at the central parts of dlx2a of 24hpf and 30hpf embryos, indicating that capsulin was expressed at mesoderm core of cranial neural crest cells. musculin was expressed at mesoderm of early stage. At the later stage, musculin was expressed at intermandibularis (im) by comparing with the expression pattern of myoD. Using morpholino knockdown approach, Our data showed that knock capsulin and musculin down led to loss of all cranial muscles. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay revealed that large amounts of cell apoptosis in the hindbrain region of capsulin-morphant and musculin-morphant, but ventral mesoderm core was not affected by capsulin MO and musculin MO. Molecular evidences showed that myf5 and myoD transcription were down-regulated at the precursor cells of head muscles in capsulin-morphant and musculin-morphant. musculin was down-regulated in myf5-morphant and myoD- morphant, whereas capsulin was not affected by myf5 MO and myoD MO. Taken together, we concluded that capsulin and musculin are required for zebrafish cranial muscles development.
| Identifer | oai:union.ndltd.org:TW/097TKU05105008 |
| Date | January 2009 |
| Creators | Gang-Hui Lee, 李耕琿 |
| Contributors | Yau-Hung Chen, 陳曜鴻 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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