碩士 / 淡江大學 / 生命科學研究所碩士班 / 97 / LMBR1 domain containing 1 protein(LMBRD1),also known as NESI,is involved in a novel nuclear export pathway.Characteristic phenotypes of the heterozygous knockout mice include stumbling,lifting the hind-legs,and dragging tails during movement suggest that LMBRD1 plays significant roles in motor coordination,learning,and memory during neuronal differentiation.To further examine its biological functions during early embryogenesis,we used zebrafish as a model system because of their well-defined developmetal stages and genetic background.We stained the embryos with a peptide antibody against human LMBRD1(αhLMBRD1) to detect the spatiotemperal expression patterns of endogenous zebrafish LMBRD1 polypeptide.At 24 hpf,expression was detected throughout the somatic mesoderm in the both fast and slow muscle populations later,zebrafish LMBRD1 was restricted in the future retina,otic placode,brain,muscle and pectoral fin buds,but were down-regulated in notochord.Transverse section through the trunk of a 72 hpf embryo showing the zebrafish LMBRD1 express on the surface of each myofibril.In the addition,zebrafish LMBRD1 express at high level in retina ganglion and brain cortex but is down-regulated in the mature muscle of adult fish.By the way,also injected the LMBRD1-morpholino into zebrafish embryos to observe the difference.The LMBRD1 knockdown fish displayed reduced head and curved-body phenotypes.To further investigate the molecular mechanisms that lead to curved-body phenotypes,we stained embryos by some muscle specific markers,such as F59,phalloidin,α-actin and dystrophin,to detect the morphological changes in muscle fiber,interactions among cytoskeletal components and membrane,respectively.Our results show that the muscle fibers of LMBRD1-morphant aligned disorderly and lost their integrity,septum curved,misalignment of motor neuron innervations,the myocardium slender.Fast and slow muscle numbers are also less than those of WT.These data suggest that LMBRD1 may function to regulate muscle fiber organization and play an important role zebrafish myogenesis.
| Identifer | oai:union.ndltd.org:TW/097TKU05105013 |
| Date | January 2009 |
| Creators | Hsin-Ju Wu, 吳欣儒 |
| Contributors | 陳曜鴻 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
Page generated in 0.0021 seconds