碩士 / 淡江大學 / 生命科學研究所碩士班 / 97 / Chitinous materials were used the sole carbon/nitrogen sources for antioxidants produced by Serratia ureilytica TKU013. To investigate the effect of different carbon/nitrogen sources (squid pen powder, shrimp head powder, crab shell powder, 0.5~2 %) on the production of antioxidant materials by TKU013, here incubated Serratia ureilytica TKU013 for 0~4 days at 25℃ and analyzed the antioxidant activity of the cultured supernatant showed using DPPH scavenging ability. Biological test that TKU013 culture supernatant (1.5 % SPP) incubated for four days has the highest antioxidant activity. Moreover, a new compound Serranticin was isolated from ethyl acetate layer.
Serranticin exhibits cytotoxic effects (MCF-7 ED50=14.78 ug/mL, WiDr ED50=6.52 ug/mL), and has strong antioxidative effects assayed by DPPH.
In the second part, Serratia sp. TKU013 was cultured using different carbon source (squid pen powder, shrimp head powder, crab shell powder, 0.5~2 %) and incubated in 50 mL of liquid media in shaking flasks at 25℃ for 0~4days, and the quantity of total polyphenol content, reducing power and Fe2+ ion chelating ability.
| Identifer | oai:union.ndltd.org:TW/097TKU05105015 |
| Date | January 2009 |
| Creators | Kao-Cheng Liu, 劉高呈 |
| Contributors | San-Lang Wang, 王三郎 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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