碩士 / 臺北醫學大學 / 醫學科學研究所 / 97 / In this study, we evaluate a novel delivery system for oral vaccines, TFP-modified liposomes entrapped in acid-induced alginate (ALT) of biodegradable polymers, which is conceived from a combination of the polymer, the lipid–based delivery system and immunostimulant. We utilized liposomes (multilamellar, MLV) of 95 % PC to encapsulate 106.0 EID50/mL inactive H5N3 virus (LV group) and coated with Tremella fuciformis polysaccharide (TFP), purified from hot water extracts of Tremella fuciformis, for 24 hr (LTV group). The virus(V), LV, LTV mixed within 1.5 % alginate with tricalcium phosphate, respectively (AV, ALV and ALTV group).
The vaccine mixed with acid-induced alginate used for in vitro release study. When HCl-induced alginate contact with HCl to forming alginate gel that was more resistant in acidic pH and modulated the release profiles of the encapsulated vaccines in the alkaline pH. Transepithelial electrical resistance (TEER) studies revealed that LV and LTV were able to opening the tight junctions (about 77.4 % and 75.4 % of the initial value, respectively) of Caco-2 cell monolayer by about three times. Recovery studies on the TEER showed that the effect of the LV and LTV vaccines on Caco-2 cell monolayer is reversible and proves the viability of cells after incubation with all vaccines.
The animal exp. showed that vaccine entrapped in alginate gel induced intestinal sIgA production at 3wk after second administration. although IgG in serum not expected to increase, and the hemagglutination inhibition test also showed that there was insufficient protection of the effectiveness of the antibody. However, there may be because it means Th1 immune suppression of the humoral immune response.
In conclusion, the TFP modified liposomal oral vaccine entrapped in HCl-induced alginate gel for improving the intestinal mucosal IgA have a certain capacity, and this type of a vaccine vector for another material improvement, and this vaccine delivery system may have potential use as a carrier for vaccine that are poorly absorbed after oral administration.
| Identifer | oai:union.ndltd.org:TW/097TMC05659004 |
| Date | January 2009 |
| Creators | Jann-Jyh Yeh, 葉展志 |
| Contributors | Shyr-Yi Lin, 林時宜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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