碩士 / 臺北醫學大學 / 醫學科學研究所 / 97 / Syndecans are a family of transmembrane proteoglycans that are known to modulate a large number of signaling pathway through the interaction with ECM (extracellular matrix) or growth factor and to regulate a great diversity of biological function. Glycosaminoglycan chains of syndecan-4 is responsible for focal adhesion formation in response to ECM coordinated integrin. Results of in vitro experiments suggest that syndecan-4 also modulate cell spreading and migration through NXIP motif of ectodomain. Syndecan-4 signaling and its role in cell adhesion is understood in mammalian cell culture systems. However, little is understood in terms of its role in development. Recently research suggested that syndecan-4 seems to play an important role in organism development. Evidence reveals syndecan-4 is involved in the signaling regulate neural induction and neural crest cells migration during early embryo development. In this study, we aim to unravel the role of syndecan-4 in zebrafish embryo development. Overexpression of syndecan-4 seems to have little defect in morphology, however, overexpression of functional motif-deleted form of syndecan-4 resulted in abnormal muscle distribution and variation of cardiovascular development. The embryo overexpressed normal or mutant form of syndecan-4 also shows the defect of lateral line system in neuraomasts. Taken together, these results suggest that syndecan-4 plays an important role in several types of cells during zebrafish development.
| Identifer | oai:union.ndltd.org:TW/097TMC05659040 |
| Date | January 2009 |
| Creators | Hao Ting, 丁豪 |
| Contributors | Chih-Ming Chou, 周志銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 44 |
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