碩士 / 中國醫藥大學 / 生態暨演化生物學研究所 / 98 / The purpose of this thesis is to assess the biokinetic (BK) and biodynamic (BD) modeling approach to chronic copper (Cu) toxicity and ecophysiological response of tilapia (Oreochromis mossambicus). We assessed the chronic toxicity that caused ecophysiological response of bioaccumulation concentrations and growth inhibition on tilapia following exposure to 0.1, 0.2, 0.3, 0.6 μg mL-1 of water-borne copper in laboratory. We conducted a 14-day exposure experiment to obtain BK parameters of uptake rate constant (k1), elimination rate constant (k2), and bioconcentration factor (BCF). The order of organ specific copper concentrations and BCF in selected organs and tissue are liver＞alimentary canal＞kidney＞gill＞muscle. Results of chronic growth toxicity assay showed that the specific growth rates (kg) are negatively correlated to waterborne Cu concentration, and EC50 (t) values in 7, 14, 21, 28 days are 0.257, 0.28, 0.269, 0.271 μg mL-1, respectively. We applied the damage assessment model (DAM) to depict the time course of toxicity and ecophysiological processes of tissue damage and recovery. We suggested that the area under the curve (AUC) was a great dose matrix for the DAM to describe the temporal trend of chronic toxicity. Alimentary canal and liver could be the sensitive target organ for assessing chronic copper toxicity. Results of this study can improve the understanding of chronic toxicities in tilapia and provide a reference for protecting the environment and aquaculture ecosystems.
| Identifer | oai:union.ndltd.org:TW/098CMCH5110011 |
| Date | January 2010 |
| Creators | Ying-Hsuan Huang, 黃映璇 |
| Contributors | Jeng-Wei Tsai, 蔡正偉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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