碩士 / 嘉南藥理科技大學 / 化妝品科技研究所 / 98 / In this study,fermentation of the Chlorella and the plants by adding two kind of bacteria in culture medium and analyzed the functional effects of fermentative metabolites.These tests included the antibacterial, antioxidative and anti-aging effects. This experiment used the Bacillus subtilis to break the contents of Chlorella and the plants into small fragments then to ferment these samples by Saccharomyces cerevisiae. The resultants were extracted with ethanol and they were detected the abilities of antibacteria, inhibiting the activity of MMPs and scavenging DPPH free radical. No significant effects of the antibacteria were found in these different extracts. In the Inhibition of MMPs, including nonfermentative and fermentative treatment of Chlorella samples showed significant abilities in inhibition of MMPs. The four samples of Chandelier plant also had obvious ability to inhibit MMPs, but nonfermentative sample had the best effect. Both the non-treated sample and the sample fermented by the SC had significant inhibition of MMP in Houseleek plant. The best sample of MMP’s activity inhibition was nonfermentative sample in Cactus plant. The inhibitory effect of MMPs activity in Aloe samples are obviously. Only nonfermentative sample could inhibit MMPs activity in Angelicakeiskei plant.On the DPPH free radical scavenging rate, the concentrations of nonfermentative samples were in 0.83,4,4,4,4mg/mL about 95,95,95,90,90％ eliminating rate in Chlorella, Chandelier, Houseleek, Cactus and Aloe plants, respectively. The concentration was in 8mg/mL, nonfermentative and fermentative sample of the Angelicakeiskei plants had about 90% DPPH scavenging rate.
| Identifer | oai:union.ndltd.org:TW/098CNUP5793025 |
| Date | January 2010 |
| Creators | Tzu-Jung Wu, 吳紫容 |
| Contributors | Kuen-Lin Leu, 呂昆霖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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