碩士 / 中臺科技大學 / 醫學工程暨材料研究所 / 98 / Abstract
Polysaccharide is the most prevalent material in the surgery. Chitosan, Sodium alginate and Hyaluronic Acid were compound with Polylactic acid fiber and absorbent cotton into a composite dressing whose biological and mechanical property were studied.
Two phases were included in this study. The optimum manufacturing parameter of the base fabric was earned in the first phase. PLA and absorbent cotton were mixed in different ratios before the mixture were processed in the following procedures: feeding, opening, channeling, carding, lamination and needle punching. Tensile strength, tear strength, moisture absorption, air permeability, vapor permeability, and flexibility of the base fabrics involving various ratios of PLA and absorbent cotton were explored.
The anti-bacterial layer and swelling layer were prepared in the second phase. Chitosan, the dominant anti-bacterial material, was spray-coated upon the base fabric chose on the basic of the result in the first phase. An anti-bacterial test was carried out on this Chitosan-coated layer. The swelling layer was prepared by crosslink-ing the sodium alginate/Hyaluronic acid solution with calcium chloride. Next, the sodium alginate/Hyaluronic acid/calcium chloride was freezing dried into a porous membrane on which cells were later cultured upon for effects of Hyaluronic acid concentrations on cell vitality. After the best Hyaluronic acid concentration was earned, swelling rate, weight loss rate, far-infrared ray, contact angle and SEM of this sodium/Hyaluronic acid/calcium chloride membrane were also examined for effects of cross-link concentrations on the membrane’s mechanical property. Finally, the optimum anti-bacterial layer and the swelling layer were compounded with the base fabric, so a composite dressing was ready for further animal tests and application evaluations.
When the PLA and absorbent cotton were mixed and processed, a base fabric with promising strength was yielded. The best mixing ratio was 8:2 (PLA: absorbent cotton). The Alg/HA membrane is firmly structured after Chitosan was cross-linked. After the Chitosan layer was immersed in the PBS for 96 hours, its average weight loss rate was under 40 %. The anti-bacterial test on staphylococcus aureus exhibited that coating the 3 wt% Chitosan in 0.154 g/cm2 brings out the best anti-bacterial effect. The average moisture permeability of this sodium alginate/ Hyaluronic acid/PLA/ absorbent cotton composite dressing was 2039±178.8 g/m2 day. According to previous studies, the result was acceptable.
The MTT cell vitality test was conducted using L929. The excellent cell survival rate was found in the HA concentration of 3 mg/ml. The Alg/HA membrane was cultured with L929 for 24 hours. The result indicated that the 0.15 % calcium chloride achieved sound cell adhesive effect. Last but not least, the animal test also confirmed that this sodium alginate/Hyaluronic acid/Chitosan/PLA/ absorbent cotton composite dressing accelerated healing.
| Identifer | oai:union.ndltd.org:TW/098CTC05530004 |
| Date | January 2010 |
| Creators | Song-lien Wu, 吳松濂 |
| Contributors | Ching-wen Lou, 樓靜文 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 139 |
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