碩士 / 中原大學 / 土木工程研究所 / 98 / Recently the researches on the CO2 fixation have been focused on the biological utilization. Microalgae will be able to use the carbon dioxide as a carbon source, produced high-density biomass, and the single cell oil extracted from biomass can be transformed into biodiesel.
This study used the high lipid content of green algae Chlorella vulgaris as the research object. Firstly, we used continuous culture to enlarge algae. Then, we set several batch experiments to control different pH, illumination and the aeration with different concentration of carbon dioxide. Through comparison of chlorophyll a, optical density, MLSS, cell number and lipid content to discuss the growth situation, we selected the most suitable condition for the growth of this algal species. We chose one-step method, which is a single-step transesterification reaction, to convert fatty acids into fatty acid methyl esters（FAME）, and then using gas chromatography (GC) to analysis esters of reaction products.
Results showed that the ratio of FAME in the extract of microalgae lipid was 76.58%, while after the one-step reaction, the ratio of FAME could raise to 94.71%. Results of batch experiment showed that alkaline environment decreased lipid content, and pH 7 was the best culture condition with algae density of 1,136.67 mg/L, growth rate of 13.2 day-1 and the total lipid content of 25.5% dry weight. The best illumination condition is 2,930 lux with accumulation of algal density of 770 mg/L, growth rate of 8.26 day-1 and total lipid content of 32.03% dry weight. It was found out that culture in high-light can accumulate the biomass of algal cells. However, growth of algae was inhibited when cultured in the 9,600 lux, and content of chlorophyll a was decreased. In CO2 batch experiments, MLSS can be accumulated up to 1,288.33 mg/L under 15% CO2 concentration of the aeration, while the maximum growth rate was 12.75 day-1. In addition, lipid accumulation the highest lipid content was 45.68% dry weight under 30% CO2 concentration of the aeration. Through batch experiments and results of lipid accumulation, this study selected pH 7, 2,930 lux, and 30% CO2 concentration as the optimal conditions for continuous culture.
| Identifer | oai:union.ndltd.org:TW/098CYCU5015020 |
| Date | January 2010 |
| Creators | Chun-Ping Su, 蘇純平 |
| Contributors | Y.T. Huang, S.J. You, 黃郁慈, 游勝傑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
Page generated in 0.0015 seconds