碩士 / 輔仁大學 / 生命科學系碩士班 / 99 / The climate change caused by greenhouse gas effect and energy shortage in the next few decades make an urgency for finding alternative energy. Of all renewable energy, biodiesel producing from microorganisms is considered as a great potential candidate. In this study a microbial isolate, TN 1, rich in oil accounting for approximately 50% of dry cell weight was obtained from Tainan coast in Taiwan. Examination of 18S rRNA, D1/D2 region of 26S rRNA, and internal transcribed spacer of rRNA genes (ITS) and analysis of short sequence by MSP-PCR, the isolate TN 1 was found to be a novel strain of yeast. The isolate TN 1 also exhibited unique patterns of nutrient utilization as well as physiological and biochemical characterizations comparing with other known yeast. After fatty acid composition analysis, TN 1 was found its to contain 17.34% of C14:0, 20.40% of C16:0, 4.69% of C18:0, 40.47% of C18:1, 11.47% of C18:2, 2.41% of C18:3, and 0.97% of C20:4. The isolate, TN 1 also prefers to grow and to generate oil in medium containing 1% seawater at 20℃ and pH 8. While using sucrose as sole carbon source, about 70% of TN 1 dry cell weight are lipids, and uses tryptone as sole nitrogen source, approximately 51% of dry weight are lipids. As the result, the novel yeast TN 1 has a great potential application in the biodiesel industry. The TN 1 will be further investigated for oil biosynthesis and for any potential applications in the near future.
| Identifer | oai:union.ndltd.org:TW/098FJU00105015 |
| Date | January 2010 |
| Creators | Hsu, Yu-Di, 許俼頔 |
| Contributors | 呂誌翼 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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