碩士 / 輔仁大學 / 營養科學系 / 98 / The infant formula is one of the essential dietary sources for the neonates. Since the liver detoxification system and renal function in the neonates have not been completely developed, high aluminum (Al) ingestion from the infant formula may cause Al accumulation which may have adverse effect on development of infants. The purposes of this study are (1) to set up an in vivo animal model of aluminum intoxication through the intragastric injection of Al, (2) to study the effect of high dietary Al on neuronal development in neonatal rat pups. The 3-day old pups were divided into three groups with intragastric injection of 0 (Control, Con), 0.6 (low Al, LAl) and 6.2 (high Al, HAl) μg Al/g b.wt/day (AlCl3) respectively within 18 days. The pups were sacrificed on the 21th day of the birth, and the serum, olfactory bulb, pituitary gland, cerebral cortex, hippocampus, thalamus, hypothalamus tissues, cerebellum and medulla oblongata were collected. Al content in brain tissues were determined by Inductively Coupled Plasma Mass Spectrometry (ICP/MS). The effects of aluminum on N-methyl-D-aspartate receptors (NMDAR) subunits 1A and 2A/B proteins expression in hippocampus and cerebral cortex of intragastric injection pups were analyzed by Western blot. The results showed that there were no significant effects of Al ingestion on body weight gains and relative organ weight, except whole brain. Al levels in various brain tissues of HAl animals were significantly higher than control groups (P<0.05). Meanwhile, the expressions of NMDAR 1A and 2A/B in hippocampus of Al-treated groups were significantly decreased, compared to the control. In conclusion, the aluminum concentration in neonatal pup’s brains was significantly increased by ingestion of aluminum, even in the Al level of infant formula on the market. While, the protein expression of the glutamatergic neuron markers related to cognition, behavior, learning and memory were significantly decreased in hippocampus with high Al intake.
| Identifer | oai:union.ndltd.org:TW/098FJU00513002 |
| Date | January 2009 |
| Creators | SHIU SHI-YI, 徐詩怡 |
| Contributors | 王果行 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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