碩士 / 輔英科技大學 / 生物技術系碩士班 / 98 / Spirulina is a microscopic blue-green algae in the shape of a perfect spiral coil living both in sea and fresh water which is the common name for human and animal food supplements produced primarily from two species of cyanobacteria: Arthrospira platensis, and Arthrospira maxima. Recent studies have demonstrated antioxidant, antiviral and anticancer effects of Spirulina extracts in cultured cells as well as in animals. In this study, we investigate the cytotoxic effects of different Spirulina extracts as well as purified phycocyanin in human myelogenous leukaemia K562 cells. The alcohol extracts shown to have the most cytotoxic from not protein components, while water extracts and phosohate buffer extracts which reserve most phycocyanin could not induced obvious apoptosis in K562 cells. We further investigated isolation and characterization of bioactive natural products from the alcohol extracts of Spirulina and found at least 11 fraction through TLC analysis which fraction number 3 was found to have the most cytotoxicity. The Protein kinase C also known as PKC is a family of enzymes that are involved in controlling the function of other proteins through the phosphorylation of hydroxyl groups of serine and threonine amino acid residues on these proteins. In this study, to investigate whether PKC signaling pathway involve in alcohol extracts induced K562 cells apoptosis pathway, the cytotoxic effects were evaluated with the administration of the PKC inhibitor staurosporine or PKC activator 12-O-tetradecanoyl-phorbol-13-acetate ( TPA) in alcohol extracts treated K562 cells. The application of PKC activator TPA upon alcohol extracts treatment blocked the alcohol extracts -induced cytotoxicity which could be abort by administration of the PKC inhibitor staurosporine. The activation of PKC can attenuate alcohol extracts induced K562 cells apoptosis. Our results indicate that PKC signaling pathway is involved in Spirulina extracts induced K562 cytotoxicity.
| Identifer | oai:union.ndltd.org:TW/098FY005108009 |
| Date | January 2010 |
| Creators | Yuan-Shyang Wang, 王元祥 |
| Contributors | Chih-Chung Chou, 周致中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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