碩士 / 國立中興大學 / 動物科學系所 / 98 / Soybean meal and fish meal are the protein source of animal diet because of higher crude protein content and with all essential amino acids needed by animal. However, the price of fish meal was more expensive because abnormal weather conditions and overfishing by people have caused the depression of fish catch. If the fish meal of diets could be replaced by SBM, the feed cost could be decreased. But, anti-nutritional factors (ANTs) of soybean meal have limited their nutritive values and utilization in diet. ANTs in soybean meal include trypsin inhibitor, flatulence-causing oligosaccharide (mainly raffinose and stachyose) and allergenic protein (β-conglycinin and glycinin). Use processing method, like heating, enzyme treatment and microorganism fermentation, could reduce some of the ANTs in soybean meal. The purpose of this study was to evaluate the feasibility of replacing the fish meal of broiler diets by two kinds of fermented soybean meal, which were inoculated SBM with Aspergillus oryzae incubated at 30℃ for 24 hours (FSBMA), followed by adding Lactobucillus casei for 24 hours fermentation at 42℃ (FSBMA+L). Both FSBMA and FSBMA+L, were dried and grounded respectively. The results showed that the crude protein, TCA-soluble protein and lactic acid content of FSBMA and FSBMA+L were increased. The flatulence-causing oligosaccharide and allergenic protein of soybean meal were depressed after fermentation. Five hundred one-day-old chicks of equal number of male and female were randomly allocated into five groups, 4 replicates of 25 chicks for 5 weeks feeding trial. The five groups included control (corn-soybean meal diet) and 4 of a half or full replacement for fish meal by FSBMA or FSBMA+L. The results indicated that chicks fed diet of FSBMA or FSBMA+L for half replacement of fish meal content didn`t be significantly different with body weight gain, feed intake and feed conversion compared with control group (P > 0.05). However, full FSBMA or FSBMA+L replacement groups significantly decreased the body weight gain and body weight at 35 day of age (P < 0.05). The pH of crop and ileum in broilers fed FSBMA and FSBMA+L was lower than in control group at 21-day-age. FSBMA+L could reduce both Coliform and Enteric bacilli population of crop and ileum at 21 day of age (P < 0.05). In addition, the fecal ammonia concentration of broilers fed FSBMA and FSBMA+L was less compared control group. In summary, FSBMA and FSBMA+L could replace a half content of fish meal in broiler diet, then the growth performance didn`t have negative effect. FSBMA+L could depress the pathogens of digestive tract and improved gut health of young broiler chicken.
| Identifer | oai:union.ndltd.org:TW/098NCHU5289014 |
| Date | January 2010 |
| Creators | Wei-Jie Wang, 王偉杰 |
| Contributors | Bi Yu, 余碧 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 69 |
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