碩士 / 國立中興大學 / 生物產業機電工程學系所 / 98 / This study was aimed at investigating the influence of elements of mediu in algae cell density increase, lipid content and fatty acid composition. The first experiment conducted was a comparison of two normal media Walne medium and f/2 medium to culture Nannochloropsis occulata. The result had shown that f/2 medium was better than Walne medium for cultivating N. occulata. To investigate the influence of elements of medium in algae cell density increase, lipid content and fatty acid composition, the ingestion of nitrate and phosphorus content was ingested in the f/2 medium over 48 hours of the testing period for each later experiment.The experimental result obtained a high negative correlation of the concentrations of nitrate and orthophosphate in culture medium with microalgae density, and the correlation coefficients were -0.905 and -0.954, respectively. The multiple linear regression analyzing result obtained a good linear relationship, and the coefficient of determination R2 was 0.909. Moreover,the fatty acid C16:1n-7 in microalgae had a high negative correlation with C18:2n-6 and C18:3n-3 during the test, and the correlation coefficients R were -0.916 and -0.965, respectively. The nitrate and orthophosphate concentrations in culture medium had a high negative correlation with fatty acid C18:2n-6 and C18:3n-3, and the correlation coefficients R achieved were -0.894 and -0.860 and -0.964 and -0.983, respectively.
In addition to the results of the first experiment, Taguchi method of S/N ratios for Larger-the-Better cases was used to test the optimization parameters for macroelements (nitrogen, phosphorus and carbon) and microelements (iron, copper, cobalt, molybdenum, manganese, zinc and magnesium) in microalgae cultivation, respectively.Macroelement testing results had shown that f/2 medium containing 22.055mg/l sodium dihydrogen phosphate (NaH2PO4) obtained the best algae cell density, lipid content,fatty acids,C18:1n-9,C18:2n-6,C20:5n-3 content and nitrate assimilation content. Microelement testing also showed that f/2 medium containing 0.0345mg/l iron chloride hexahydrate (FeCl3•6H2O) obtained the best algae cell density, fatty acid C16:1n-7,C20:5n-3 content and assimilation orthophosphate content. Furthermore, f/2 medium containing 0.27mg/l manganese chloride tetrahydrate (MnCl2•4H2O) also obtained a considerable amount of lipid content production. The study suggests that further research and explorations are needed to gain a more accurate test results; past research studies and other references on microalgae cultivated medium for industry production can also be added in future studies to provide better comparisons and analysis.
| Identifer | oai:union.ndltd.org:TW/098NCHU5415022 |
| Date | January 2010 |
| Creators | Chih-wei Chen, 陳志瑋 |
| Contributors | 尤瓊琦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 108 |
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