碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 98 / In Asia, shrimp culture is an important industry. Over the last two decades, however, outbreaks of viral diseases have led to devastating financial loss. One such disease, white spot syndrome (WSS), is caused by the white spot syndrome virus (WSSV). In order to develop disease prevention strategies it is important to understand host-pathogen interactions. These are poorly known in WSSV. One line of research focuses on shrimp proteins that respond to infection. These proteins are identified, selected and their function is investigated. Previous research using two-dimensional gel electrophoresis (2-DE) found that one such protein, 14-3-3, was up-regulated in two shrimp viral diseases (WSS and Taura syndrome). This is a highly conserved protein, found in all eukaryotic organisms. It functions as a helical dimer for the regulation of many signal transduction pathways. In this study, the 14-3-3 unique to Litopenaeus vannamei was identified and its function during WSSV infection was explored. This protein, named Lv14-3-3, is comprised of a 741-bp open reading frame encoding a polypeptide of 246 amino acids. Phylogenetic analysis found that all 3 known shrimp 14-3-3s formed a distinct subclade within the invertebrate clade. Lv14-3-3 mRNA expression was found in most shrimp tissue. Indirect immunofluorescence showed that it was primarily found in the cytoplasm of the cell, rather than in nuclei. After WSSV infection, mRNA and protein expression levels increased. Lv14-3-3 was activated through phosphorylation by protein kinase, previous studies showed that protein kinase phosphorylated 14-3-3 alters subsequent signal responses because of a change of binding affinity between 14-3-3 and its ligands. It is therefore possible that WSSV triggers a signal transduction mechanism. Lv14-3-3 therefore acts as an adapter protein which triggers subsequent responses during WSS infection. Further research could identify specific 14-3-3 host responses and identify key host cellular factors which support the WSSV lifecycle within host cells. Such cellular factors offer hope for the design of anti-WSSV drugs.
| Identifer | oai:union.ndltd.org:TW/098NCKU5111120 |
| Date | January 2010 |
| Creators | Hao-ShuoChang, 張豪碩 |
| Contributors | Han-Ching Wang, 王涵青 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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