碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 98 / It has recently been suggested that Dscam (Down syndrome cell adhesion molecule), a member of the immunoglobulin-superfamily (IgSF), plays an essential role in the adaptive immune system of invertebrates. Here, we isolated and characterized the first shrimp Dscam from Litopenaeus vannamei. The LvDscam protein had an extracellular domain but lacked the expected transmembrane domain and cytoplasmic tail, both of which are found in all other members of the Dscam family (and may also be found in other L. vannamei Dscams that have not yet been isolated). In nervous tissue, expression levels of LvDscam were unexpectedly low. Phylogenetic analysis suggests that LvDscam is far from the Dscams found in other invertebrates. Nevertheless, the domain architecture of the extracellular region of LvDscam is similar to other invertebrate Dscams, and it exhibits the typical configuration of 10 immunoglobulin (Ig) domains, 6 fibronectin type 3 domains (FNIII) and one cell attachment sequence (RGD). Cloning and characterization of a total of 62 cDNAs from hemocytes collected from WSSV-free, WSSV-persistent and WSSV–acute-infected shrimp revealed 23 alternative amino acid sequences in the N-terminal of Ig2, 30 in the N-terminal of Ig3 and 13 in the Ig7 domain. This implies that LvDscam can potentially encode at least 8,970 unique isoforms. Further analysis suggested that the LvDscam Ig2 and Ig3 regions are more functionally important than Ig7 in the shrimp’s specific immune response against WSSV. We discuss how this tail-less, soluble Dscam can still play an active role in alternative adaptive immune response even while its axonal guidance functionality may be impaired.
Identifer | oai:union.ndltd.org:TW/098NCKU5111121 |
Date | January 2010 |
Creators | Pin-HsiangChou, 周品翔 |
Contributors | Han-Ching Wang, 王涵青 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | en_US |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 39 |
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