碩士 / 國立東華大學 / 海洋生物科技研究所 / 98 / Intracellular lipid bodies (LBs) have been identified as the pivotal organelle regulating the endosymbiotic association between corals and dinoflagellates ( Luo et al. 2009). The biogenesis of LBs, as examined by a dual-emission ratiometric analysis in live cells, is dependent on the symbiotic status of corals. In the present study, the research focus extrapolated from the level of cell to the tissue and organism in order to understand how the dynamics of LB population is regulated in polyps of the Euphyllia glabrescens. First, fixed tentacles were incubated with osmic textroxide (OsO4). Distribution and density of LBs were quantified after serial cryo-sections by the imaging analysis. Results showed that the LB population in the gastrodermis was under a diel regulation, which reached the maximum at the sunset and then gradually diminished before sunrise. Furthermore, there was a directional re-distribution of the LB population across the gastroderm during the daily fluctuation cycle. Lipid analysis of the LB population indicated that there were consistent dynamics of several lipid species, including the wax ester/ triglycerides and cholesterol ester. However only wax ester concentration had daily cycle change. LB gradually diminished before sunrise may be it release to tentacle lumen at night. The present study has provided important insight, showing that the LB formation is an important biological rhythm that may directly regulate reef formation.
| Identifer | oai:union.ndltd.org:TW/098NDHU5270011 |
| Date | January 2010 |
| Creators | Hui-Jung Kang, 康惠珺 |
| Contributors | Chii-Shiarng Chen, 陳啟祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 128 |
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