碩士 / 國立東華大學 / 海洋生物科技研究所 / 98 / Marine invertebrates often form the obligate symbiosis with microalgae Symbiodinium, such as the endosymbiosis during the Cnidaria-Symbiodinium association. The cellular and molecular mechanism of the endosymbiosis remains unclear. The aim of the present study is to study how the division or Symbiosinium in endosymbiosis is regulated by examining the role of cAMP in cell cycle regulation. Results showed that dynamic level of intracellular cAMP correlated with cell cycle propagation in cultured Symbiodinium. During the 12 hours-light and 12 hours-dark light-dark cycle, the [cAMP]i oscillated with two peakings at periods when cells entered S stage (from G1 stage) and G1 stage (from G2/M stage), respectively. The oscillation of [cAMP]i was diminished when cells were exposed to continual dark treatment. When cells were treated with continual light irradiation, significant [cAMP]i fluctuation were significant. Pretreated Symbiodinium with IBMX, which inhibition cAMP degradation, retarded cell cycle.
| Identifer | oai:union.ndltd.org:TW/098NDHU5270015 |
| Date | January 2010 |
| Creators | Chia-Jung Li, 李佳蓉 |
| Contributors | Chii-Shiarng Chen, 陳啟祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 109 |
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