碩士 / 國立高雄海洋科技大學 / 水產養殖研究所 / 98 / The objectives were to investigate the transcript levels of G protein-coupled receptor 54 (gpr54), mammlian gonadotropin releasing hormone (mgnrh), tyrosine hydroxylase (th), monoamine oxidase (mao), dopamine D2 receptors (d2ar and d2br), gnrh receptor and gonadotrpins (fsh-β and lh-β) after catfish pituitary homogenates (CPH) or salmon pituitary homogenates (SPH) treatments in female Japanese eels. Furthermore, we also investigated the mRNA expression levels of wild female eels and cultured female eels. Our results showed that GSI was increased after CPH or SPH treatments, but the SPH had a significant effect. 17β-estradiol (E2) and testosterone (T) levels of serums were increased in CPH or SPH treated eels. The gpr54, mgnrh, mao, d2ar and gnrh receptor transcript levels in brain and pituitary were no significantly difference between controls and CPH treated eels. SPH significantly decreased gpr54 transcript levels in pituitary. The mgnrh transcript levels of forebrain, midbrain and pituitary were significant increased in SPH treated eels. The th transcript levels of forebrain significantly increased in GSI≒6% group, but there were no significantly changed in midbrain after CPH treatments. CPH significantly decreased d2br transcript levels in forebrain. th mRNA expression levels forebrain and midbrain were not significantly different in SPH treated eels as compared to the control. The d2ar and d2br transcript levels of forebrain were significantly increased in SPH treated eels. The mao and gnrh receptor transcript levels were not significantly different between control and SPH treatments. fsh-β transcript levels of pituitary were decreased in CPH or SPH treated eels. In contrast, lh-β transcript levels of pituitary were significantly increased in CPH or SPH treated eels. GSI in the wild female eels was 0.18~2.6 %. The concentrations of E2 and T in serum were significantly increased with GSI. In wild eels, gpr54 transcript levels in forebrain were negatively correlated with GSI. mgnrh, th, d2ar and gnrh receptor transcript levels in brain and pituitary were not significantly correlated with GSI. d2br transcript levels in forebrain and midbrain were significantly decreased with the increase of GSI. The transcript levels of mao and fsh-β and lh-β in pituitary were significantly correlated with the increase of GSI. The culture eels were all at the previtellogenic stage and the GSI range was 0.15～1.17 %. Therefore, the gene expression levels were not significantly changed in brain and pituitary in this study. In conclusion, our data showed that there is kiss/GPR54 system in eels and GPR54 may not play as a stimulator in the reproduction of eels. We also found that dopamine did have strong inhibitory effects in Japanese eels.
| Identifer | oai:union.ndltd.org:TW/098NKIM8086001 |
| Date | January 2010 |
| Creators | Yi-Ting Pen, 潘怡婷 |
| Contributors | Shan-Ru Jeng, 鄭絢如 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 117 |
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