碩士 / 國立高雄海洋科技大學 / 水產養殖研究所 / 99 / The main purpose of this paper is selected lactic acid bacteria in aquatic animal research and for future applications. Bacteria isolated from fish intestinal, according to lactic acid bacteria of characteristics as Bergey's Manual described to isolated . Using Escherichia coli, Streptococcus sp., Samolnella sp., Pseudomonas aeruginosa and Bacillus subtilis in suppression test. Selected from the inhibition of lactic acid bacteria isolated in better one, and testing the growth promoting effect of fish. The experiment was divided into four parts. First experiment: The selective medium MRS (Mann Rogosa and Sharp) separation of the intestinal tract of cultured fish species, use the biochemical tests and protein electrophoresis to summarized the composition proteins of isolated bacteria, and identificated by 16s ribosomal RNA. Biochemical characteristics of lactic acid bacteria strains were screened 69 strains, according to type of protein composition and induction with Lactobacillus plantarum, Lactobacillus sakei, Leuconostoc holzapfelii, Pediococcus acidilactici, Pediococcus pentosaceus, Pediococcus stilesii, Weissella cibaria, Weissella confusa, Weissella paramesenteroides, Weissella viridescens 10 different groups of lactic acid bacteria species, and which the distribution of P. acidilactici as most bacteria.
Experiment II: the isolated lactic acid bacteria in TSA (Tryptic Soy Agar) in the anchor zone of inhibition test paper for E. coli, Streptococcus sp., Salmonella sp., Pseudomonas aeruginosa and B. subtilis. For antibacterial activity test, the results of 981101mfia2– W. confusa, 981213spi1– W. paramesenteroides. The inhibition of 980727mfia1– P. acidilactici was the most excellent of Streptococcus sp., Salmonella sp., Pseudomonas aeruginosa. To B. subtilis, The inhibitory effect of P. acidilactici most significant; W. confusa and W. paramesenteroides of Streptococcus sp. and Salmonella sp. effect is more significant.
Experiment III: the pathogenic test, intraperitoneal injection to tilapia 981101mfia2, 981213spi1 and 980727mfia1 of 109 cfu /0.2 ml per fish of bacteria and control group were injected saline. Experient for 7 days, the results showed that the mixture of all bacterium injection treatment not fatal and not cause stress reactions as loss of appetite or vitality.
Experiment IV: promoting the growth experiment, fish were feeding 20 % in 981101mfia2、981213spi1 and 980727mfia1 of the 109cfu bacilli, such as volume mixed MRS broth of control. After four weeks, the assessment of the feed conversion rate (Feed Conversion Ratio, FCR) and (Specific Growth Rate, SGR). Add lactic acid bacteria group can increased of feeding behavior and feeding volume, the all three treatment groups in the SGR and FCR values were also significantly increased, and 981101mfia2 and 981213spi1 were most excellent.
| Identifer | oai:union.ndltd.org:TW/098NKIM8086003 |
| Date | January 2010 |
| Creators | Huang shih che, 黃詩哲 |
| Contributors | 張朴性 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 117 |
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