碩士 / 國立澎湖科技大學 / 食品科學研究所 / 98 / Giant Grouper (Epinephelus lanceolatus) and Cobia (Rachycentron canadum) are high-valued fish in Taiwan, and this study was use RAPD (Random amplified polymorphic DNA) and ISSR (Inter simple sequence repeat) of molecular marker technique to analyze genetic diversity of E. lanceolatus and R. canadum. The samples were collected from wild and cultured populations of Penghu, Pingtung and Kaohsiung in Taiwan. All samples were confirmed to the species and extracted DNAs were analyzed for COI gene sequences, and then 95 primers of RAPD and 59 primers of ISSR were used to identify the genetic diversity for all samples. Results showed that COI gene sequences be differentiated wild and cultivated populations of E. lanceolatus, and the RAPD method was that 21 primers were produced 279 bands, and obtained 86 polymorphic bands (31% of polymorphism), the RAPD 115 primer was produced high of polymorphic bands, with RAPD 73 could be discriminated wild and cultivated populations of E. lanceolatus; 17 of ISSR primers (29.3 % of tatol primers) were produced 166 bands, and obtain 58 polymorphic bands (34.9 % of polymorphism), the ISSR 868, ISSR 813, ISSR 22 and ISSR 2 (60 %) primers were produced more polymorphic bands. To analyzing the pattern of the RAPD and ISSR method, wild and cultivated populations of E. lanceolatus could be discriminated, and then using SCAR (Sequence characterized amplified region) of molecular marker to differentiate Serranidae fish and E. lanceolatus fish, the results were shown that E. lanceolatus fish could be amplified the target fragment 310 bp, but Serranidae fish didn’t have positive reaction. SCAR could be an useful method for identification of fish. In this study , those methods could not discriminated genetic diversity between the wild and cultured populations of R. canadum. However, this study could provide the molecular marker technology for the aquaculture industry applications. By using RAPD analysis live fish scales which we collected have the same results as sampling from muscle. In this study, the COI gene and DNA moleculer marker were developed and could be provide information for variety identification, clonal propagation, and genetic improvement of species-specific in aquacultues.
| Identifer | oai:union.ndltd.org:TW/098NPHT7252004 |
| Date | January 2010 |
| Creators | Pai, Chu-Ying, 白珠穎 |
| Contributors | Chiu, Tsai-Hsin, 邱采新 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 160 |
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