碩士 / 國立屏東科技大學 / 水產養殖系所 / 98 / The objectives of this study were to investigate the effects of different levels and forms of selenium (Se) on the growth performance, antioxidant response, and immunity and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii. Sodium selenate (SSe) and seleno-L-methionine (MSe) were used as inorganic and organic Se source, respectively. Five experimental diets, including a control diet (without Se supplement), 0.5 and 1 mg/kg of MSe, 0.5 mg/kg and 1 mg/kg of SSe, were used. Providing the actual dietary values of control, 0.5, 1 mg/kg MSe supplied diet and 0.5, 1 mg/kg SSe supplied diet were 1.17±0.07 mg/kg, 1.59±0.21 mg/kg, 2.22±0.45 mg/kg, 1.53±1.36 mg/kg and 2.19±0.86 mg/kg, respectively. Prawns were fed with the experimental diets for 77 days. The growth performance trial showed that a basal diet containing about 1.17 mg/kg of Se is sufficient for growth. The prawn fed the Se supplied diets had greater antioxidant enzyme activities, including superoxide dismutase, glutathione peroxidase, glutathione reductase, and glutathione S-transferase compared to those fed the control diet. In the trail of the immunity of prawn fed with Se supplied diets, no significant difference in total haemocyte count, superoxide dismutase and clearance efficiency were found among all treatments and control. However, phenoloxidase activity, respiratory burst, glutathione peroxidase, glutathione reductase, glutathione S-transferase and phagocytic activity of prawn fed with both forms of Se containing diets (organic Se and inorganic Se) were significant increased. For challenge test, prawn fed with two forms of Se containing diets at the level of 0.5 mg/kg has a significant lower mortality compared to the control prawn after being injected with Debaryomyces hansenii. It was suggest that feed contain with Se results in 1.5 mg/kg Se in diet may enhance prawn antioxidant status, immunity and disease resistance, and the basal diet containing 1 mg/kg of Se is enough for prawn growth.
| Identifer | oai:union.ndltd.org:TW/098NPUS5086007 |
| Date | January 2010 |
| Creators | Shun-Ji Jian, 江順吉 |
| Contributors | Chun-Hung Liu, 劉俊宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
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