碩士 / 國立屏東科技大學 / 水產養殖系所 / 98 / Two Varicorhinus species, V. barbatulus and V. alticorpus, inhabit rivers of Taiwan. One of them, V. barbatulus, has become a high-economic fish on this island in recent years. Cytogenetic studies allowed an understanding of the basic patterns of karyotypes in these Varicorhinus species. The information will be available for genetic studies and breeding of these species in the future. In this study, we applied different staining methods including Giemsa, Ag-NOR, C-banding, and the fluorescence in situ hybridization (FISH) technique using 5S and 18S ribosomal DNA probes to analyze karyotypic variations of the two species. Our results showed that V. barbatulus and V. alticorpus have the same chromosome number: 2n = 50. Karyotypic formulas (KFs) and fundamental numbers (FNs) of females and males of V. barbatulus were 10 m + 24 sm + 16 a (FN = 84) and 10 m + 23 sm + 17 a (FN = 83), respectively. In contrast, KFs and FNs of females and males of V. alticorpus were 6 m + 26 sm + 18 a (FN = 82) and 6 m + 27 sm + 17 a (FN = 83), respectively. These results showed significant differences in karyotypic patterns between the two species, but male individuals of both species possessed a pair of heterochromosomes. However, their sex determination system should be defined as the XX/XY system. In addition, data from the Ag-NOR analysis also expressed different cytogenetic characterizations and can be used to distinguish these two Taiwanese Varicorhinus species.
| Identifer | oai:union.ndltd.org:TW/098NPUS5086017 |
| Date | January 2010 |
| Creators | Nian-Cih Chen, 陳念慈 |
| Contributors | Mei-Chen Tseng, 曾美珍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 55 |
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