碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 98 / This study investigated the immunological protection by applying injectable vaccines of Vibrio alginolyticus strain S4Y and yolk IgY against the same vaccines on juvenile grouper. The bacterium originally isolated from diseased groupers was cultured and passed in the fish three times to increase its virulence, and then was used in this study for experimental purposes including vaccine preparation and virulence test. The LD50 value of the bacterium in grouper was 2.0×104 CFU/g fish body weight.
For active immunization, the best protective groups were group Inject II (using soybean oil as adjuvant supplemented with lipopolysaccharide) and Oral (encapsulated with agarose) with a same survival rate after immunization for 4, 8, 12 and 16 weeks prior to a lethal bacterial challenge. Of those, the survival rate were 100, 100, 100 and 75%, respectively. Following the best two protective groups was group Inject I (adjuvanted with soybean oil only) with survival rate of 100, 100, 100 and 75%, respectively. Meanwhile, Only a little protection was achieved in group Bath after immunization for 4 weeks with a survival rate of 50%. In contrast, no protection was achieved in group Dip after immunization.
For passive immunization, the group fed with 2% egg yolk IgY against V. alginolyticus bacterium in feed daily for one week and two weeks exhibited the best protection with survival rates of 100 and 90%, respectively. However, the increase of the IgY against the bacterium to 4% in feed did not enhance the proportion with survival rates of 20 and 20%, respectively. The additional of 1% glucan in the 2% IgY in feed did not exhibit better performance with survival rates of 100 and 80%, respectively. In addition, fish fed with 2% and 4% IgY against divalent bacterium (V. alginolyticus S4Y and V. carchariae EMI) in feed exhibited good protection with survival rates of 70 and 100%, 80 and 100%, respectively. The results indicated that the additional of 2% IgY against V. alginolyticus challenge.
In conclusion, the present results reveal that the use of soybean oil as adjuvant supplemented with lipopolysaccharide and the use of agarose for encapsulation in feed possess potential for further development in injectable vaccine and oral vaccine, respectively. Furthermore, the additional of 2% IgY against V. alginolyticus on divalent bacterin exhibits good potential for passive immune protection against the vibriosis in grouper fry.
| Identifer | oai:union.ndltd.org:TW/098NTOU5086029 |
| Date | January 2010 |
| Creators | Po-Tsang Lee, 李柏蒼 |
| Contributors | Kuo-Kau Lee, 李國誥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 109 |
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